植物生长调节剂和基质种类对杉木无性系瓶外生根组培苗质量的影响

Effect of Exogenous Hormone and Media Type on Quality of Ex Vitro Rooting Subculture Plantlet of Cunninghamia lanceolata

以杉木无性系组培苗为材料,采用正交设计和单形重心混料试验设计,研究不同植物生长调节剂和基质种类及配比对苗木质量的影响。结果表明:植物生长调节剂浓度对杉木无性系组培继代芽苗瓶外生根率和根数量的影响较大,以100 mg/L IBA溶液浸泡芽苗1 h的组合方式生根效果最佳,平均生根率89.56%。基质种类和配比方式对继代芽苗瓶外生根率和苗木生长均有较大影响,移栽基质的成分应以泥炭土和蛭石为主,珍珠岩和红心土可不加或少量添加。总根长、总根表面积、总根体积、平均根直径等根系形态参数是评价杉木无性系瓶外生根组培苗质量的重要形态指标。利用隶属函数值对不同基质配方的苗木质量进行综合评价,同时兼顾生根率指标,筛选出7号配方为较适宜的移栽基质,该配方由泥炭土和蛭石按照体积比0.6∶0.4混合而成,移栽2个月后苗木生根率为79.91%,移栽12个月后平均苗高为23.80 cm,平均地径为4.09 mm,而且根系发达,苗木综合质量明显优于红心土中的对照苗木。

The trial was designed by orthogonal design and simplex centroid mixture to find out the effects of different exogenous hormone and media on quality of Cunninghamia lanceolate clones.The results showed that the concentration of exogenous hormone had a great influence on the ex vitro rooting rate and root number of subculture plantlet buds of C.lanceolate clones.The combination of soaking buds in 100 mg/L IBA solution for 1 hour was the best solution,with an average ex vitro rooting rate of 89.56%.The composition and proportion of media had significant effects on ex vitro rooting rate and quality.Peat soil and vermiculite should be the main components of transplanting media.On the contrary,pearlite and red mud should not be added or added as little as possible.Root morphological parameters,including total root length,total root surface area,total root volume and average root diameter,are important indices for evaluating the quality of ex vitro rooting subculture plantlet of C.lanceolate.The quality of C.lanceolate clone was comprehensively evaluated by subordinate function values,and the ex vitro rooting rate was also taken as the important index.And then,No.7 media,contained 60%peat soil and 40%vermiculite,was selected as the best media.Cultivated in No.7 media for 2 months,the ex vitro rooting rate was 79.91%.After 12 months of cultivation,average height and ground diameter was 23.80 cm and 4.09 mm,respectively.And root architecture and comprehensive quality of C.lanceolate clones were better than that cultivated in red mud.