非消化寡糖对小鼠免疫调节的影响

Effects of non-digestible oligosaccharides and their combinations on the immunomodulation of mice

为探讨低聚果糖、低聚木糖和低聚半乳糖3种非消化寡糖及其组合对小鼠的免疫调节作用,将140只雌性BALB/c小鼠随机分为7组(空白对照组、低聚果糖组、低聚木糖组、低聚半乳糖组、低聚木糖与低聚半乳糖复合组、低聚木糖与低聚果糖复合组、低聚半乳糖与低聚果糖复合组),各组分别以400 mg/kg的剂量进行灌胃,每天1次,连续15 d。通过测定免疫器官指数、巨噬细胞吞噬能力、血清溶血素水平、DTH迟发型变态反应程度、脾淋巴细胞增殖水平等指标,评价不同非消化寡糖及其组合对小鼠的免疫调节作用。结果显示,单一寡糖灌胃组与组合寡糖灌胃组小鼠各项免疫指标均显著(P<0.05)或极显著(P<0.01)高于对照组;组合寡糖灌胃组小鼠各项免疫指标均高于单一寡糖灌胃组,其中低聚木糖与低聚半乳糖(2 3,质量比)复合灌胃组效果最好。以上研究结果表明,非消化寡糖能够增强小鼠的免疫应答能力且对其进行组合有更好的效果。

To investigate the immunomodulatory effects of the three non-digestible oligosaccharides,fructooligofructose,xylooligosaccharide and galactooligosaccharide,and their combinations on mice,140 BALB/c female mice were randomly divided into 7 groups in this experiment,including blank control group,FOS group,XOS group and GOS group,XOS and GOS group,XOS and FOS group,FOS and GOS group.Each group was intragastrically administered at a dose of 400 mg/kg once a day for 15 days.The immunomodulatory effects of different non-digested oligosaccharides and their combinations on mice were evaluated by measuring immune organ index,macrophage phagocytosis,serum hemolysin level,DTH delayed type hypersensitivity,and spleen lymphocyte proliferation.The results showed that the immunological indexes of the single oligosaccharide-administered group and the combined oligosaccharide-administered group were significantly higher(P<0.05 or P<0.01)than the control group.The immune index of combined oligosaccharide gavage group rats was higher than the single oligosaccharide gavage group,and the combination of xylooligosaccharide and galacto-oligosaccharide(1 1.5)was the best.The above results indicated that non-digestible oligosaccharides could enhance the immune response ability of mice and the combination of them had better effect.