处理方式对米根霉菌Rho18清除呕吐毒素的影响

Effect of treatment on the elimination of deoxynivalenol by Rho18

通过酶联免疫吸附测定法(ELISA)及高效液相色谱-质谱联用仪(HPLC-MS)测定不同方式处理的米根霉菌Rho 18对小麦粉中呕吐毒素(deoxynivalenol,DON)的清除效果,探讨米根霉菌对DON的清除能力。结果显示,米根霉菌Rho 18对小麦粉中DON的清除率均随浓度增高及孵育时间的延长而提高,各高浓度处理组孵育10 h后与其他处理组均存在显著差异,高浓度的细胞壁悬液与小麦粉孵育10 h条件下对DON的清除效果最好(51.62%)。不同方式处理的米根霉菌Rho 18与DON作用后产生3种相对分子质量在277,279,281左右的新物质。结果表明,不同方式处理的米根霉菌Rho 18对DON均有一定清除作用,且作用效果与浓度和孵育时间相关,其清除机制主要为生物酶系作用。

The effect of Rho 18 treated with different methods on the removal of deoxynivalenol(DON)from wheat flour was determined by ELISA and HPLC-MS.Processing results showed that the different ways of rice root mildew Rho18 clearance to DON in wheat flour were all increased with the concentration increase with the extension of incubation time,as well as the high concentration of the treatment group.After 10 h incubation with the rest of the concentration of treatment group were significantly different,the cell walls of a high concentration of suspension and wheat 10 h incubation conditions to DON removal effect is best(51.62%).Three kinds of new substances with relative molecular weight of 277,279 and 281 were produced by Rho 18 and DON treated in different ways.The results showed that Rho 18 of Rhizopus oryzae treated in different ways had certain scavenging effects on DON,and the effect was related to concentration and incubation time,and its scavenging mechanism was mainly the action of biological enzyme system.