成品卷烟霉变微生物的分离纯化与鉴定

Isolation,purification and identification of microorganisms in moldy cigarettes products

从霉变成品卷烟中分离、纯化得到5种菌株,并采用真菌形态学和显微镜检的方法进行鉴定。结果表明,引起卷烟霉变的微生物主要为曲霉菌、青霉菌、链格孢菌和枝孢菌;通过18S rDNA-ITS序列分析及构建系统进化树,鉴定为5个种,分别为聚多曲霉、黄曲霉、极细枝孢霉、指状青霉和链格孢属霉菌;通过致霉性试验得出黄曲霉菌为导致卷烟霉变的主要菌株。

Five strains were isolated and purified from moldy cigarettes,and were identified by fungal morphology and microscopy.It was found that there were 2 strains of Aspergillus,1 strain of Penicillium,1 strain of Alternaria,and 1 strain of Cladosporium.Through the 18S rDNA-ITS sequence analysis and the construction of phylogenetic tree,the mildew microorganism of cigarettes were identified as five species,such as Aspergillus sydowii,Aspergillus flavus,Cladosporiumtenuissimum,Penicilliumdigitatum and Alternaria.It was concluded that Aspergillus flavus was the main strain causing mildew in cigarette products by the mildew experiment.