摘要
从霉变成品卷烟中分离、纯化得到5种菌株,并采用真菌形态学和显微镜检的方法进行鉴定。结果表明,引起卷烟霉变的微生物主要为曲霉菌、青霉菌、链格孢菌和枝孢菌;通过18S rDNA-ITS序列分析及构建系统进化树,鉴定为5个种,分别为聚多曲霉、黄曲霉、极细枝孢霉、指状青霉和链格孢属霉菌;通过致霉性试验得出黄曲霉菌为导致卷烟霉变的主要菌株。
Five strains were isolated and purified from moldy cigarettes,and were identified by fungal morphology and microscopy.It was found that there were 2 strains of Aspergillus,1 strain of Penicillium,1 strain of Alternaria,and 1 strain of Cladosporium.Through the 18S rDNA-ITS sequence analysis and the construction of phylogenetic tree,the mildew microorganism of cigarettes were identified as five species,such as Aspergillus sydowii,Aspergillus flavus,Cladosporiumtenuissimum,Penicilliumdigitatum and Alternaria.It was concluded that Aspergillus flavus was the main strain causing mildew in cigarette products by the mildew experiment.
作者
雷声
杨乾栩
师艳萍
蒋举兴
刘秀明
张玲
陈文怡
汪雪娇
曾熠程
LEI Sheng;YANG Qian-xu;SHI Yan-ping;JIANG Ju-xing;LIU Xiu-ming;ZHANG Ling;CHEN Wen-yi;WANG Xue-jiao;ZENG Yi-cheng(Technology Center,China Tobacco Yunnan Industrial Co.,Ltd.,Kunming,Yunnan 650231,China;School of Food Science and Technology,Jiangnan University,Wuxi,Jiangsu 214122,China)
出处
《食品与机械》
北大核心
2019年第9期98-101,共4页
Food and Machinery
基金
云南中烟工业有限责任公司科技项目(编号:2017CP06)
国家“十三五”重点研发计划(编号:2017YFD0400105)
关键词
霉变卷烟
微生物
分离
鉴定
黄曲霉
mildew cigarette
microorganism
separation
identification
Aspergillus flavus