不同产地甜橙果皮提取物抗氧化活性成分及能力研究

Study on antioxidant components and antioxidant activities of peels extracts from sweet orange in Hunan province

以8种不同产地甜橙皮为研究对象,比较其抗氧化活性成分及抗氧化能力。研究结果表明,麻阳冰糖橙皮中多酚、黄酮、单宁和V C含量最高,其含量分别为7.60 mg GAE/g·样品干重、4.57 mg RE/g·样品干重、17.90 mg TAE/g·样品干重、17.86 mg/g·样品干重。同时,麻阳冰糖橙皮也展现了最高的铁离子还原能力[(81.50±0.93)μmol TE/g·样品干重]、很高的DPPH·清除能力[(15.77±0.64)μmol TE/g·样品干重]和ABTS·清除能力[(77.70±0.75)μmol TE/g·样品干重]。Spearman相关分析表明,这4类成分含量与抗氧化活性具有显著相关性。高效液相色谱鉴定甜橙皮中的5种主要黄酮类化合物为橙皮苷、柚皮苷、橙皮素、川陈皮素和桔红素,其中以橙皮苷含量最高[(2.63±0.21)^(4.65±0.33)mg/g·样品干重]。主成分分析和层次聚类分析将8种甜橙分为三类,第Ⅰ类:永兴冰糖橙、洪江冰糖橙、麻阳冰糖橙和浏阳脐橙;第Ⅱ类:石门冰糖橙和石门脐橙;第Ⅲ类:麻阳脐橙和邵阳脐橙。尤其以第Ⅰ类的多酚、黄酮、单宁和V C含量高,铁离子还原能力和ABTS·清除能力较强。甜橙皮中抗氧化活性物质的含量及其抗氧化活性可以作为区分不同产地甜橙的重要指标。

Eight kinds of sweet orange peels extracts were investigated to compare their antioxidant active ingredients and antioxidant capacity.The results showed that the highest level of the content of polyphenols,flavonoids,tannins and vitamin C were found in Mayang Bingtang sweet orange peel extracts,which reached 7.60 mg gallic acid equivalent(GAE)/per gram dried sample(g·m d),4.57 mg rutin equivalent(RE)/g·m d,17.90 tannin(TAE)mg/g·m d,17.86 mg vitamin c(VC)/g·m d,respectively.Meanwhile,Mayang Bingtang orange peel also showed the highest ferric reducing power(81.50±0.93μmol Trolox equivalent(TE)/g·m d),and very high 1,1-diphenyl-2-trinitrophenylhydrazine(1,1-diphenyl)-2-picrylhydrazyl(DPPH)free radical scavenging capacity(15.77±0.64μmol Trolox/g·m d)and 2′-hydrazine-bis-3-ethylbenzothiazoline-6-sulfonic acid(2,2′-azino-Bis(3-ethylbenzothiazoline-6-sulfonic acid),(ABTS)free radical scavenging ability(77.70±0.75μmol Trolox/g·m d).Spearman correlation analysis showed that the contents of polyphenols,flavonoids,tannins and vitamin C were significantly correlated with antioxidant activity.Five major flavonoids such as hesperidin,naringin,hesperetin,nobiletin and tangeretin in orange peel were identified by high performance liquid chromatography,and the highest content was hesperidin(2.63±0.21~4.65±0.33 mg/g·m d).Principal component analysis and hierarchical cluster analysis results showed that the eight kinds of sweet oranges could be divided into three groups.The first group include Yongxing Bingtang orange(A),Hongjiang Bingtang orange(B),Mayang Bingtang orange(D)and Liuyang navel orange(H),and the second group include Shimen Bingtang orange(C)and Shimen navel orange(E),and the third group consisted of Mayang navel orange(F)and Shaoyang navel orange(G).Especially in the first group rich in polyphenols,flavonoids,tannins,vitamin C content and high FRAP and ABTS radical scavenging capacity.The antioxidants content and antioxidant activity of sweet orange peel can be used as an important index to distinguish sweet orange from different producing areas.