湖南部分地区奶牛乳房炎主要病原菌的分离鉴定及药敏试验

Isolation,Identification and Drug Susceptibility Test for the Main Pathogenic Bacteria of Cow Mastitis in Some Local Areas of Hunan

为了解湖南省部分地区奶牛乳房炎主要病原菌种类及耐药情况,同时制定科学合理的用药方案,对来自不同地区的74份奶样进行细菌分离鉴定,并对其主要病原菌进行药敏试验。结果显示,该地区奶牛乳房炎奶样中葡萄球菌、大肠杆菌、乳房链球菌、棒状杆菌的分离比例分别为47.72%、18.18%、13.64%、10.23%,其他菌种仅占10.23%。对其中检出率较高的葡萄球菌、大肠杆菌和乳房链球菌进行药敏试验,结果显示3种病原菌对头孢拉啶和恩诺沙星高度敏感,对链霉素和磺胺异噁唑有较强的耐药性;葡萄球菌和大肠杆菌对青霉素和氨苄西林有较强的耐药性;乳房链球菌对四环素有较强的耐药性。因此,头孢拉啶和恩诺沙星可以作为该地区奶牛乳房炎治疗的优选药物。

The article is aimed to investgate the main pathogen species of cow mastitis,study the drug resistance situation and finally to formulate a scientific and reasonable medication regimen in some local areas of Hunan.Totally 74 mastitis milk samples from different areas were collected for bacterial isolation and identification test.Results showed that,in these areas,the main bacterial pathogen for dairy cow mastitis were S.aureus(47.72%),Escherichia coli(18.18%),Strepotococcus uberis(13.64%)and Corynebacterium(10.23%),other strains accounted for only 10.23%.Drug susceptibility testing showed that S.aureus,E.coli and Strepotococcusuberis were highly sensitive to cefradine and enoxacin but had strong resistance to Streptomycin and sulfafurazole Staphylococcus and Escherichia coli were highly resistant to penicillin and ampicillin.Strepotococcus uberis had strong resistance to tetracycline.Therefore,cefradine and enoxacin can be used as the preferred drugs for the treatment of dairy cow mastitis in these areas.