在线富集与高效液相色谱-荧光检测法联用同时检测儿童水杯中9种双酚类物质

Determination of nine bisphenols in children’s water bottle by online enrichment coupled with high performance liquid chromatography-fluorescence detection

基于在线富集-高效液相色谱-荧光法检测法,以C 18柱分别作为富集柱和分析柱,经优化荧光波长、色谱分离和富集条件,建立了同时快速检测塑料儿童水杯浸出液中9种双酚类物质的方法。方法学考察结果显示,所建方法符合含量测定要求。9种双酚类物质在各自范围内线性关系良好,相关系数(r^2)均大于0.998,检出限为0.13~66.7 ng/L。9种双酚类物质的回收率为90.7%~112.4%(RSD<11.3%,n=6)。结果显示,除双酚芴外,其他双酚类物质均有检出,浸出量随盛放时间增加而增大,高温多次浸泡后部分双酚类浸出量降低。该方法灵敏度高,操作简便,环境友好,可实现多种新型双酚类物质的同时快速检测。

A method was developed for the rapid determination of bisphenol A(BPA)and eight structural analogs in children’s plastic water bottles by online enrichment coupled with high performance liquid chromatography-fluorescence detection(HPLC-FLD).The correlation coefficients of the nine bisphenols were greater than 0.998.The limits of detection(LOQs)ranged from 0.13 ng/L to 66.7 ng/L.The recoveries ranged from 90.7%to 112.4%(RSD<11.3%,n=6).This method was applied to monitor nine bisphenols in children’s water bottles.The results showed that except 4,4′-(9 H-fluoren-9-ylidene)bisphenol(BPFL),all the remaining eight bisphenols were detected in different water bottles.The amounts of bisphenols leached increased with the increase of soaking time,and decreased after washing several times at 100℃.The proposed strategy is rapid,sensitive,reliable and eco-friendly,and is suitable for the simultaneous analysis of new bisphenols in water samples.