仙人掌多糖对卵巢癌大鼠癌组织细胞凋亡的干预作用

Effect of Opuntia dillenii Polysaccharide on Apoptosis of Ovarian Cancer Tissue in Rats

为探讨仙人掌多糖对卵巢癌大鼠癌组织细胞凋亡的干预作用,选取了50只SD健康雌性大鼠,其中10只作为正常组,其余40只建立卵巢癌模型并分为模型组和低、中、高浓度仙人掌组,对各组大鼠分别进行干预,观察各组大鼠癌组织细胞凋亡以及细胞周期分布情况,并对Bcl-2、PI3K、Akt、PTEN、p-Akt、mTOR蛋白表达进行检测。研究结果表明,高浓度仙人掌组肿瘤体积、重量分别为(179.63±51.82)V/mm^3、(0.30±0.05)W/g,低于其他各组;高浓度仙人掌组细胞凋亡率及G1期细胞比例分别为19.22%、62.33%,高于其他各组;高浓度仙人掌组大鼠Bcl-2、PI3K、Akt、p-Akt、mTOR相对表达量分别为1.37、1.45、1.40、1.35、1.39,均低于模型组及低、中浓度仙人掌组;PTEN相对表达量为0.76±0.05,显著高于模型组及低、中浓度仙人掌组(p<0.05)。在仙人掌多糖的干预下,卵巢癌大鼠癌组织细胞凋亡率上升,细胞周期受到阻滞,凋亡相关蛋白Bcl-2、PI3K、Akt及周期蛋白PTEN、p-Akt、mTOR表达受到调控,其能力呈现浓度依赖,为卵巢癌的临床治疗提供一定的理论帮助。

To investigate the effect of Opuntia polysaccharide on apoptosis of ovarian cancer rats,fifty SD healthy female rats were selected,of which 10 were normal group,the remaining 40 were used to establish ovarian cancer model and divided into model group and low,medium and high concentration cactus group.The rats in each group were intervened to observe the apoptosis and cell cycle distribution of cancer tissue.The expression of Bcl-2,PI3K,Akt,PTEN,p-Akt and mTOR protein were also detected.The results showed that the volume and weight of tumors in high concentration cactus group were 179.63±51.82 V/mm^3 and 0.30±0.05 W/g,respectively,which were lower than those in other groups.The apoptotic rate and G1 phase cell proportion in high concentration cactus group were 19.22%and 62.33%,respectively,which were higher than those in other groups.The relative expression scores of Bcl-2,PI3K,Akt,p-Akt and mTOR in high concentration cactus group were higher than those in other groups,which were 1.37,1.45,1.40,1.35,1.39,respectively.The relative expression of PTEN was 0.76,which was higher than that of model group and low and medium concentration cactus group(p<0.05).Under the intervention of Opuntia polysaccharide,the apoptotic rate of ovarian cancer model rats increased and cell cycle was blocked.The expression of apoptotic related proteins Bcl-2,PI3K,Akt and cyclin PTEN,p-Akt and mTOR were regulated,and their ability was concentration-dependent.It could provide some theoretical guideline for the clinical treatment of ovarian cancer.