壳聚糖醋酸盐的制备及其油脂吸附性能研究

The Preparation of Chitosan Acetate Salt and Its Fat-Binding Capacity

采用半湿研磨法制备了壳聚糖醋酸盐(Ch-Ac),对其结构、理化性质进行了表征和测定。以食用油为吸附对象,探讨了搅拌速度、温度、介质pH等因素对Ch-Ac油脂吸附性能的影响,通过正交实验确定了Ch-Ac油脂吸附的最佳条件。实验结果表明:(1)Ch-Ac改善了壳聚糖的溶解性能而且具有较强的缓冲能力;(2)搅拌速度1000 r/min、温度25℃、介质pH=5的条件下,Ch-Ac的油脂吸附量(OBC)高达168.72 g/g,是壳聚糖OBC的10倍;(3)在pH=2~10的介质中,Ch-Ac的OBC均大于133.23 g/g,模拟人体胃肠pH环境连续变化,Ch-Ac的OBC为148.13 g/g。结果表明Ch-Ac的油脂吸附能力受介质pH影响较小。固体Ch-Ac制备方法简单、绿色环保,油脂吸附效率高,可在含油废水处理和降脂保健品等方面得到应用。

Chitosan acetate(Ch-Ac)was prepared by a semi-wet grinding method.The structure and physicochemical properties of Ch-Ac were characterized and determined.Using edible oil as the adsorption target,the effects of stirring speed,temperature and medium pH on the oil-binding capacity(OBC)of Ch-Ac were investigated.Basing on orthogonal experiments,the optimum conditions for the binding of oil by Ch-Ac were established.The experimental results were as follows:(1)The solubility of chitosan was improved in the form of Ch-Ac and Ch-Ac exhibited a strong buffering capacity;(2)The OBC of Ch-Ac was up to 168.72 g/g,which was 10 times as high as that of chitosan,at a stirring speed of 1000 r/min,absorption temperature of 25℃and pH 5.(3)The OBC of Ch-Ac was greater than 133.23 g/g in a medium with a pH of 2~10,while reaching 148.13 g/g in a simulated human gastrointestinal environment with a continually changing pH.These results indicated that the OBC of Ch-Ac was less affected by the medium pH.The preparation method of solid Ch-Ac is simple,green and environmentally friendly.The obtained Ch-Ac has high OBC thus can be applied to treat oil-containing waste water and develop lipid-lowering health care products.