西洋参醇提物对慢性萎缩性胃炎大鼠的逆转效应研究

Reversal Effect of American Ginseng Alcohol Extracts on Rats with Chronic Atrophic Gastritis

目的探讨西洋参醇提物对亚硝基胍(N-methyl-N’-nitro-N-nitrosoguanidine,MNNG)诱发慢性萎缩性胃炎(chronic atrophic gastritis,CAG)模型大鼠的萎缩逆转作用及对PCNA蛋白、Bcl-2蛋白、PCNA mRNA、Bcl-2 mRNA、TFF3 mRNA、TNF-αmRNA的影响。方法Wistar大鼠自由饮用浓度为167μg mL的MNNG饮用液,实验造模12周。确定成模后,将大鼠分为模型组、正常组、西洋参醇提物组。并分别给予药物干预后,观察大鼠体质量,胃组织病理组织学,血清胃泌素(gastrin,GAS),胃组织PCNA蛋白、Bcl-2蛋白、PCNA mRNA、Bcl-2 mRNA、TFF3 mRNA、TNF-αmRNA表达情况。结果治疗第8周西洋参醇提物组体质量恢复显著高于模型组(P<0.05)。西洋参醇提物组腺体萎缩、不典型增生、肠化显著减轻(P<0.01)。西洋参醇提物组炎症等级、GAS较模型组无显著性差异。西洋参醇提物组PCNA蛋白、Bcl-2蛋白较模型组显著降低(P<0.01)。西洋参醇提物组PCNA mRNA、TFF3 mRNA、TNF-αmRNA较模型组均有下降,但差异无统计学意义;Bcl-2 mRNA较模型组有显著升高(P<0.01)。结论西洋参醇提物对CAG模型大鼠具有良好的治疗作用,减轻体质量丢失,减轻胃黏膜萎缩和肠化生,其作用机制之一可能是通过下调胃黏膜PCNA蛋白、Bcl-2蛋白的高表达,起到改善乃至逆转CAG病理状态的作用。

OBJECTIVE To discuss the reverse effect of gastric atrophy and the influences to PCNA and Bcl-2 protein,PCNA mRNA,Bcl-2 mRNA,TFF3 mRNA,TNF-αmRNA of American Ginseng alcohol extracts for the treatment of the model rats with chronic atrophic gastritis(CAG)induced by N-methyl-N’-nitro-N-nitrosoguanidine(MNNG).METHODS Wistar rats were free to drink MNNG solution with a concentration of 167μg·mL-1,and they were made models for 12 weeks.After making the model successfully,divided the rats into model group,normal group and American Ginseng alcohol extracts group.After giving drugs intervention separately,observed the weight of rats,gastric tissue histopathology,gastrin(GAS),the expression of PCNA and Bcl-2 protein and mRNA,TFF3,TNF-αmRNA in gastric tissue.RESULTS In the eighth therapeutic week,the weight of rats in the American Ginseng alcohol extracts group were significantly higher than the model group(P<0.05).The glandular atrophy,atypical hyperplasia and intestinal metaplasia were significantly relieved in the American Ginseng alcohol extracts group(P<0.01).There was no significant difference of gastric mucosa inflammation level GAS between the American Ginseng alcohol extracts group and the model group.There were significant reduce of content of the PCNA protein and Bcl-2 protein in the American Ginseng alcohol extracts group(P<0.01).In the American Ginseng alcohol extracts group,there was a decline of PCNA mRNA,TFF3 mRNA and TNF-αmRNA but had not significant,Bcl-2 mRNA was significantly increased compared with model group(P<0.01).CONCLUSION American Ginseng alcohol extracts have a good therapeutic effect on the CAG model rats in reducing weight loss and reducing gastric atrophy and intestinal metaplasia,one of the mechanism may be down regulating the high expression of PCNA and Bcl-2 in gastric mucosa,and to improve and even reverse the pathological state of CAG.