桑黄水煎液对D-半乳糖衰老模型小鼠的抗氧化作用

Effect of Sanghuang Decoction on Oxidative Damage Induced by D-galactose in Mice

目的观察桑黄水煎液对D-半乳糖诱导小鼠氧化损伤的保护作用,并探讨其保护作用的可能机制。方法①ICR小鼠分为正常对照组,桑黄水煎液低、中、高剂量(2,6,12 g·kg^-1)组,连续给药15 d。②ICR小鼠分为正常对照组、模型组、维生素E组(50 mg·kg^-1),桑黄水煎液低、中、高剂量(2,6,12 g·kg^-1)组,小鼠颈背部皮下注射D-半乳糖(120 mg·kg^-1)造成亚急性衰老模型,按分组连续给药42 d。比色法测定小鼠肝脏及血清中丙二醛(MDA)含量和抗氧化酶活力,如超氧化合物歧化酶(SOD)、总抗氧化能力(T-AOC)、过氧化酶(POD)等。Western blot检测肝脏中核因子E2相关因子2(Nrf2)和血红素氧合酶1(HO-1)的表达。结果桑黄水煎液对正常小鼠和D-半乳糖诱导小鼠体质量及脏器指数均无明显影响。桑黄水煎液可显著提高正常小鼠肝脏中T-AOC和SOD酶活力(P<0.01,P<0.05)。各剂量桑黄水煎液可显著提高衰老模型小鼠血清中T-AOC,降低MDA含量(P<0.01,P<0.05),同时中剂量增强肝脏中SOD(P<0.05)和POD酶活力(P<0.01),中、高剂量均上调肝脏中Nrf2和HO-1蛋白表达水平(P<0.01)。结论桑黄水煎液具有提高正常小鼠抗氧化能力和改善D-半乳糖致小鼠氧化损伤作用,此作用可能是通过调节Nrf2/HO-1途径增加体内抗氧化酶的活性,减少过氧化脂质的生成,进而提高机体抗氧化能力。

OBJECTIVE To observe the protective effect of Sanghuang decoction on oxidative damage induced by D-galactose in mice and explore the possible mechanism.METHODS①The mice were divided into the normal group,the Sanghuang decoction low,middle,high dose(2,6,12 g·kg^-1)group,and administrated for 15 days.②Mice were divided into the normal group,model group,vitamin E(50 mg·kg-1)group,Sanghuang decoction low,middle,high dose(2,6,12 g·kg^-1)group.Subacute aging model was induced by subcutaneous injection of D-galactose(120 mg·kg^-1)into the neck and back of mice.The mice were continuous administrated for 42 d.The content of malondialdehyde(MDA)and activity of antioxidant enzymes such as superoxide dismutase(SOD),total antioxidant capacity(T-AOC)and peroxidase(POD)were measured by colorimetry in the liver and serum of mice.The levels of nuclear factor E2-related factor 2(Nrf2)and heme oxygenase 1(HO-1)proteins in liver were detected by Western blot.RESULTS Sanghuang decoction had no significant effect on body weight and organ index of normal mice and D-galactose induced mice.Sanghuang decoction could significantly improve the activity of T-AOC and SOD in the liver of normal mice(P<0.01,P<0.05).Sanghuang decoction could significantly increase T-AOC and reduce MDA content in serum of mice with aging model(P<0.01,P<0.05),meanwhile middle dose enhanced the activities of SOD(P<0.05)and POD(P<0.01),and middle and high dose upregulated the levels of Nrf2 and HO-1 proteins in liver of mice with aging model(P<0.01,P<0.05).CONCLUSION Sanghuang decoction can enhance the antioxidant capacity of normal mice and improve the oxidative damage induced by D-galactose,which maybe increase the activity of antioxidant enzymes in vivo,reduce the generation of lipid peroxide and improve the antioxidant capacity via Nrf2/HO-1 pathway.