摘要
The response of glutathione S-transferase(GST)in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature.Specific speaking,express ZmGST in Escherichia coli,then purify the recombinant protein and make the thermal stability analysis.Therefore,the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z.marina.In conclusion,the thermostability and the response of ZmGST gene to temperature changes can determine its temperature tolerance range,and affect its resilience in turn.
The response of glutathione S-transferase(GST) in Zostera marina to temperature variation was analyzed at molecular level by cloning the microsomal GST gene and texting the microsomal GST expression regularity under different temperature. Specific speaking, express Zm GST in Escherichia coli, then purify the recombinant protein and make the thermal stability analysis. Therefore, the experiments were carried out to provide a theoretical basis for the further elaboration to the population degradation mechanisms of Z. marina. In conclusion, the thermostability and the response of Zm GST gene to temperature changes can determine its temperature tolerance range, and affect its resilience in turn.
基金
The Open Fund of Key Laboratory of Marine Spil Oil Identification and Damage Assessment Technology,State Oceanic Administration under contract No.201704
the Shandong Provincial Natural Science Foundation of China under contract No.ZR2018MD020
