组学在花药发育研究中的应用进展Ⅱ:蛋白质组学和代谢组学

Application Progress of Omics in the Research of Anther Development Ⅱ: Proteomics and Metabonomics

自人类基因组计划开展以来,在迅速发展的各种测序和鉴定技术的推动下,已经得到了许多物种的基因组、转录组、蛋白组和代谢组序列、表达、修饰或代谢信息。目前人们已经意识到,任何基因或蛋白都很难单独地行使某种功能,各基因或蛋白间的相互作用是它们调节或参与生命活动的基本方式。另外,花药发育是植物进行有性生殖最重要的两个过程之一,其中与花粉发育和释放有关的各种发育事件的顺利进行是有性生殖成功的关键。目前随着组学技术的兴起,各种组学技术也已经广泛地应用于花药发育方面的研究工作。其中,从中心法则上看,蛋白质和许多生物小分子是生命活动的直接承担者,因此利用蛋白质组学和代谢组学技术对植物花药进行大规模地深入研究将进一步扩展并深化人们对花药发育机制的理解和应用。总结了植物花药和花粉的蛋白质组学和代谢组学的研究进展,分别阐述了蛋白质组学在不同研究方向的应用价值和特点,为相关基础和应用研究提供了可借鉴的理论依据。

Since the launch of the Human Genome Project,with the rapid development of various sequencing and identification techniques,people have obtained the information of sequence,expression,modification or metabolic for genome,transcriptome,proteome and metabolome in many species.At present,researchers have realized that it is difficult for any gene or protein to function independently.The interaction between genes or proteins is the basic way for them to regulate or participate in life activities.In addition,anther development is one of the two most important processes in plant sexual reproduction,and the successful development of various developmental events related to pollen development and release is the key to the success of sexual reproduction.At present,with the rise of omics technology,various omics techniques have also been widely used in the research of anther development.Among them,from the genetic central dogma,protein and many small biomolecules are the direct bearers of life activities.Therefore,large-scale in-depth study of plant anthers by proteomics and metabolomics will further expand and deepen people's understanding and application of anther development mechanism.In this paper,we summarized the progress of proteomics and metabolomics of plant anthers and pollens,and elaborated the application value and characteristics of proteomics in different research fields,providing a useful theoretical basis for related basic and applied research.