EDTA抗凝标本保存条件和时间对微柱凝胶法检测血型的影响

Influence of Different Preservation Condition and Period on Blood Grouping by Micro Column Gel Method about EDTA Anticoagulant Specimen

目的探讨不同保存条件下保存时间对微柱凝胶法检测血型的影响。方法随机选取体检健康人群128例EDTA-K2抗凝标本室温保存,另128例EDTA-K2抗凝标本4℃保存,观察开始溶血和完全溶血时间,对未溶血标本、部分溶血和完全溶血标本分别用微柱凝胶法进行血型鉴定。结果EDTA抗凝标本在室温和4℃条件下保存1周均无溶血发生;第14天时,常温保存标本约有1/3开始溶血,4℃保存标本只有少量发生溶血;常温保存标本第35天时均完全溶血,而4℃保存标本第77天时均完全溶血。标本未溶血时,正反定型结果均清晰可见;标本发生部分溶血时,正定型结果无影响,反定型结果有影响;标本完全溶血时,正反定型结果均有严重影响。结论在室温和4℃条件下,EDTA抗凝标本的最佳保存时间为1周,此时用微柱凝胶法检测血型不受影响;常温标本保存35天内、4℃标本保存77天内,对反定型有影响,此时用微柱凝胶法检测血型须谨慎;常温标本保存超过35天、4℃标本保存超过77天则不宜用微柱凝胶法检测血型。

Objective To investigate the effect of different preservation period under different storage temperatures on blood grouping by micro column gel.Methods Stochastic healthy 128 EDTA-K2 anticoagulation specimens were preserved at room temperature,while the other 128 EDTA-K2 anticoagulation specimens were stored at 4℃.The time of initial hemolysis and complete hemolysis was observed and blood group was identified on each specimen for non-hemolysed,partial hemolysed and completely hemolysed,respectively,using micro column gel method.Results No hemolysis occurred for both specimens stored at room temperature and 4℃within one week.On the 14th day,one third specimens of room temperature preservation started to hemolyse,however only a small amount of 4℃preserved specimens hemolysed.It took 35 days for all specimens stored at room temperature to completely hemolyse and 77 days for those stored at 4℃.Forward and reverse grouping results were clearly visible on non-hemolysed specimens.No impact observed on forward grouping results but there was impact on reverse grouping,in partial hemolysed specimens.Complete hemolysis affect both forward and reverse blood typing results seriously.Conclusion At room temperature and 4℃,the best preservation period for EDTA anticoagulant specimen is one week,and the detection of blood type by micro column gel method is not affected.For specimens preserved at room temperature within 35 days and 4℃within 77 days,the results show impact on reverse grouping.The determination of blood group using micro column gel method on these preservation conditions should be cautious.Specimens of room temperature preservation over 35 days and specimens preserved at 4℃more than 77 days are unfavorable for blood grouping with micro column gel method.