水稻耐低温基因COLD1功能标记的开发及应用

Development and Application of Functional Marker of Chilling Tolerance Gene, COLD1, in Rice

水稻起源于热带和亚热带地区,因此水稻对低温胁迫比其它农作物更加敏感。低温可以影响水稻生长发育的各个时期。特别是在温带和高纬度地区,低温冷害是影响水稻生产的重要环境因素之一。培育种植耐低温的水稻品种是解决低温冷害的一个重要手段。水稻耐冷基因COLD1编码一个G蛋白信号调节因子,该基因中起源于中国野生稻的SNP2位点,使粳稻具有更强的耐寒性。本研究根据COLD1中SNP2的序列多态性,开发与耐低温基因COLD1共显性的dCAPS分子标记,并利用7个水稻品种和96个籼粳交F2个体验证标记的准确性。实验结果证明本研究中设计的dCAPS分子标记能准确的鉴定粳稻COLD1^jap基因型、籼稻COLD1ind基因型及杂合基因型,可应用分子标记辅助育种工作。

Rice,evolved in tropical and subtropical regions,is more sensitive to chilling stress than other crops.Low temperature,has the potential to affect rice growth and development at any developmental stage,is one of primary environmental factors limiting rice production,especially in temperate and high altitude areas.The rice chilling tolerance gene,COLD1,encodes a regulator of G protein signaling.The SNP2 of COLD1 derived from Chinese Oryza rufipogon confers chilling tolerance in japonica rice.In this study,a dCAPS marker,codominants to the COLD1,was developed according to the polymorphism of SNP2 in COLD1.The accuracy of the marker was verified in 7 rice varieties and 96 individuals of a F2 population derived from a cross between japonica and indica.The result showed that the dCAPS marker designed in this study could accurately identify the genotypes of homozygote of japonica COLD1 japand indica COLD1 indand heterozygote COLD1 jap/COLD1 ind,and could be applied to molecular marker-assisted breeding.