黑果枸杞(Lycium ruthenium)种子萌发特性与抗旱性

Seed Germination Characteristics and Drought Resistance of Lycium ruthenium

种子萌发是植物生长过程的重要阶段,水分胁迫是影响种子萌发的重要因素之一,会对植物的生长、形态结构和产量等产生重要影响。为了研究水分胁迫对黑果枸杞萌发特性的影响,本试验设轻度水分胁迫(10%PEG)、中度水分胁迫(15%PEG)及重度水分胁迫(20%PEG)、浓度渗透PEG(6000)溶液以模拟水分胁迫条件,并设零浓度PEG(6000)的CK组,对17份采自不同种源的黑果枸杞供试材料萌发特性及其抗旱性进行了研究。结果表明:20%PEG会对17个供试材料的发芽率、发芽势、活力指数、发芽指数、相对根长、抗旱指数有较大程度的抑制;15%PEG居中;10%PEG抑制程度则较小,各指标较CK均随水分胁迫的增加而显著降低(p<0.05)。胚根/胚轴的比值在3种不同程度的胁迫下均增加,表现为20%PEG>15%PEG>10%PEG>CK。采用抗旱隶属函数值的方法对上述7个指标的耐旱性进行综合评价,2号、5号为强抗旱类型;6号为中等抗旱;16号、15号、10号、11号、12号、17号、1号、3号、8号、14号、13号、4号、7号和9号为弱抗旱材料。上述结果既可为保护抗旱植物资源及荒漠地区植被提供基础科学依据,又可为黑果枸杞育苗种植及推广提供参考价值。

Se ed germination is an important stage in the process of plant growth.Water stress is one of the important factors affecting seed germination,which will have an important influence on plant growth,morphology structure and yield.The aim of our study is to study the effects of water stress on germination characteristics of Lycium ruthenium.This test by mild water stress(10%PEG),moderate water stress(15%PEG)and severe water stress(20%PEG)concentration in the penetration PEG(6000)solution simulated water stress condition,and a zero concentration PEG(6000)of CK group,the germination and drought resistance of 17 Lycium ruthenium different species were studied.The results showed that the 20%PEG could inhibit the germination rate,germination potential,vigor index,germination index,relative root length and drought resistance index of 17 experimental materials.The 15%PEG centered;the 10%PEG was less inhibited,and each indicator decreased significantly with the increase of water stress(p<0.05).The ratio of the radicle/hypocotyl was increased under three different degrees of stress.Drought-resistant subordinate function value method is adopted for comprehensive evaluation of the above seven indexes of drought resistance,the results showed that No.2,No.5 were drought-resistant;while No.6 were moderate drought-resistant;experimental materials of No.16,No.15,No.10,No.11,No.2,No.7,No.3,No.14,No.3,No.7 and No.9 were weak drought-resistant.This study not only provide scientific basis for protecting drought-res istant plant resources and cultivating vegetation in desert areas,but also provide reference value for seedling cultivation and promotion of Lycium barbarum.