三七总皂苷对大鼠骨骼肌损伤后纤维瘢痕和血清前列腺素E2的影响

The Effects of Panax Notoginseng Saponins Injection on Muscle Fiber Scar,Serum PGE2 in a Rat Skeletal Muscle Injury Model

目的:探讨大鼠骨骼肌急性钝挫伤后,应用三七总皂苷对肌组织纤维瘢痕形成和血清中前列腺素E2含量的影响。方法:78只雄性SD大鼠,其中72只左侧腓肠肌中段建立钝挫伤模型后随机分为药物组和对照组(各36只),另6只不做处理(标记为空白组);药物组动物模型建立后即刻在损伤部位皮下注射三七总皂苷100 mg/kg,对照组注射等量生理盐水,每2 d注射1次,直至损伤后的第14天。药物组和对照组在损伤后即刻及第4,7,14,28和42天收取标本,空白组在第42天时取材。采用Masson染色观察肌肉损伤处纤维瘢痕的形成,利用Image pro plus 6.0软件计算瘢痕的面积,并对大鼠血清PGE2的含量进行检测。结果:药物组纤维瘢痕面积在各时间点均明显小于生理盐水对照组;药物组血清PGE2含量的下降速度较对照组要快,至第7天及第14天明显少于生理盐水对照组。结论:三七总皂苷通过减少大鼠骨骼肌修复过程中纤维瘢痕面积的形成,同时降低血清PGE2的含量,可能起到抗炎镇痛的作用,从而提高大鼠骨骼肌损伤后修复的质量。

Objective:The aim of this study was to investigate the effects of Panax notoginseng saponins on muscle fiber scar, serum prostaglandin E2(PGE2)after acute blunt trauma in rat skeletal muscle.Methods:78 male Sprague-Dawley rats, 72 of which had a model of blunt contusion in the middle of the left gastrocnemius muscle, were randomly divided into a drug group and a control group, and the other 6 were not treated and they were marked as a blank group.After the establishment of the animal model of the drug group, the Panax notoginseng saponin injection was injected subcutaneously at the injury site immediately 100 mg/kg, and the control group was injected with the same amount of normal saline, once every 2 d until the 14 th day after the injury.The drug group and the control group received the specimen immediately after the injury, 4, 7, 14, 28, and 42 d, and the blank group was taken at the 42 d.Masson staining was used to observe the formation of fibrous scars in muscle lesions.The area of scar was calculated by Image pro plus 6.0 software, and the serum PGE2 was detected.Results:The scar area of the drug group was significantly smaller than that of control group.The rate of descent of serum PGE2 in drug group was faster than that of control group, and obviously less than that of control group at day 7 and day 14.Conclusion:Panax notoginseng saponins can reduce the formation of scar area in the repair process after skeletal muscle injury in a rat model, and may play an anti-inflammatory and analgesic role by reducing the content of serum PGE2, thereby improving the quality of repair after skeletal muscle injury in rats.