补肾通络方对肺纤维化大鼠碱性成纤维细胞生长因子表达的影响

Effect of bushen tongluo formula on the expression of basic fibroblast growth factor in the rats with pulmonary fibrosis

目的观察补肾通络方对肺纤维化大鼠肺组织碱性成纤维细胞生长因子(bFGF)表达的影响。方法采用博来霉素诱导建立实验性肺纤维化大鼠模型,以氢化可的松琥珀酸钠为对照,观察补肾通络方对肺纤维化大鼠肺组织病理形态及肺组织bFGF和bFGF mRNA表达的影响。结果同正常组比较,模型组大鼠肺组织病理纤维化显著,炎性反应明显,同时伴有肺组织bFGF和bFGF mRNA表达升高(P<0.05,P<0.01)。同模型组相比,补肾通络方高剂量组和氢化可的松琥珀酸钠对照组大鼠肺组织bFGF和bFGF mRNA表达明显下降(P<0.05,P<0.01),肺组织病理形态与模型组比较也得到改善。结论补肾通络方可能通过从蛋白和基因水平调节实验性肺纤维化大鼠肺组织bFGF表达,抑制肺纤维化。

Objective To observe the effect of bushen tongluo formula on the expression of basic fibroblast growth factor(bFGF)in the rats with pulmonary fibrosis.Methods The rat models of experimental pulmonary fibrosis were prepared by bleomycin induction.Hydrocortisone sodium succinate was used as the control.The pathological morphology of lung tissue and the expressions of bFGF and bFGF mRNA were observed in the rats with pulmonary fibrosis.Results Compared with the normal group,the pathological fibrosis was significant and the inflammatory reaction was obvious,besides the expressions of bFGF and bFGF mRNA were increased in the model group(P<0.05,P<0.01).Compared with the model group,the expressions of bFGF and bFGF mRNA were reduced obviously in the high-dose group of bushen tongluo formula and the control group of hydrocortisone sodium succinate(P<0.05,P<0.01),and the pathological morphology of lung tissue was improved.Conclusion Bushen tongluo formula inhibits pulmonary fibrosis probably by regulating the levels of protein and gene and bFGF expressions of lung tissue in the rats with experimental pulmonary fibrosis.