基于GEO数据库分析非酒精性脂肪肝关键分子机制

Analysis of the Key Molecular Mechanisms in Non-alcoholic Fatty Liver Disease Based on the Gene Expression Omnibus Database

目的:探讨非酒精性脂肪肝中关键分子机制。方法:在GEO数据库下载数据GSE89632进行分析。将筛选出的差异基因(DEGs)输入DAVID数据库进行GO及KEGG富集分析,用Cytoscape软件构建PPI互作分析图,用Cytohubba软件求得10个靶基因。结果:通过筛选后发现124个DEGs(103个上调基因及21个下调基因)。经过DAVID分析后发现TNF信号通路、JAK/STAT信号通路等参与NAFLD的发生发展。结论:JUN、IL6、FOS、MYC、CCL2、IL8、IL1B、ATF3、EGR1、SERPINE1等10个过表达靶基因可作为脂肪肝的潜在生物学标记物,TNF信号通路可能是其关键作用分子机制。

Objective:To explore the key molecular mechanisms in non-alcoholic fatty liver disease(NAFLD).Methods:Microarray data were downloaded for GSE89632 from the gene expression omnibus(GEO)database,Functional and pathway enrichment analyses were performed by the DAVID database,and the protein–protein interaction network was constructed with Cytoscape software.In addition,Cytohubba software was used to calculate and analyze the first 10 target genes.Results:In total,124 differentially expressed genes(DEGs)were identified,including 103 upregulated and 21 downregulated genes.DAVID analyses showed that TNF signaling pathway,JAK/STAT signaling pathway and other biological processes are involved in the occurrence and development of fatty liver.Conclusion:In 124 DEGs,JUN,IL6,FOS,MYC,CCL2,IL8,IL1B,ATF3,EGR1,SERPINE1 were target genes and they can be used as potential biological markers for NAFLD.TNF signaling pathway might play the crucial role in process of NAFLD.