基于生理指标早期诊断异丙隆对小麦药害的研究

Early diagnosis of isoproturon phytotoxicity on wheat seedlings based on physiological indices

为了早期诊断异丙隆对小麦的药害,采用室内生测的方法测定了不同剂量异丙隆作用下小麦株高、地上部鲜重、叶绿素、丙二醛(MDA)、可溶性糖、脯氨酸(Pro)含量和最大光量子产量(Fv/Fm)。结果表明,除可溶性糖外,叶绿素、MDA、Pro、Fv/Fm均能快速响应异丙隆对小麦的胁迫。当50%异丙隆WP施用剂量≥3 000 g/hm^2时,叶绿素a、b、总叶绿素含量和Fv/Fm明显下降,Pro含量明显上升,与清水对照相比均达显著水平,MDA含量随着施药剂量的增加而逐渐上升,当剂量≥2 250 g/hm ^2时,小麦MDA含量显著高于清水对照处理;叶绿素、MDA、Pro、F v/F m可作为诊断异丙隆对小麦药害的敏感指标。

For early diagnosis of isoproturon phytotoxicity on wheat seedlings,the plant height,shoot fresh weight,chlorophyll,malondialdehyde(MDA),soluble sugar,proline(Pro)content and Fv/Fm were measured by dose-response experiments in the glasshouse.The results showed that chlorophyll,MDA,Pro content and Fv/Fm except soluble sugar could rapidly respond to isoproturon stress in wheat seedlings.After being treated with isoproturon 50%WP at 3 000 g/hm^2 or more,chlorophyll content and Fv/Fm decreased significantly and Pro content increased significantly.The MDA content gradually increased with the increase of isoproturon dosage,after being treated with isoproturon at 2 250 g/hm^2 or more;the MDA content was significantly higher than the control.Chlorophyll,MDA,Pro,Fv/Fm can be used as sensitive indicators for early diagnosis of isoproturon phytotoxicity on wheat seedlings.