磁共振T1rho技术定量诊断大鼠肝纤维化

Value of T1rho in quantitative diagnosis of liver fibrosis in rats

目的探讨T1rho技术定量诊断大鼠肝纤维化的价值。方法选取45只SD大鼠为实验组,以腹腔注射40%CCl 4橄榄油溶液的方法制备肝纤维化模型;另选取12只SD大鼠为对照组,腹腔注射等量生理盐水。于给药后第1、2、4、6、8、10周分别取实验组5~6只大鼠和对照组2只大鼠进行MR T1rho扫描。完成检查后取肝脏进行病理检查,并进行肝纤维化分级。结果肝纤维化F0~F6级大鼠肝脏的T1rho值分别为(40.68±1.60)ms、(42.80±1.08)ms、(44.98±1.04)ms、(46.47±3.47)ms、(47.73±3.84)ms、(55.38±7.46)ms及(57.08±5.11)ms,总体差异有统计学意义(F=30.72,P<0.01)。大鼠肝脏T1rho测量值与肝纤维化分级呈正相关(r=0.88,P<0.01);α-SMA表达水平与肝纤维化分级呈正相关(r=0.74,P<0.01),与T1rho值亦呈正相关(r=0.57,P<0.01)。结论MR T1rho可用于定量、无创性评估大鼠肝纤维化程度。

Objective To investigate the value of T1rho in quantitative diagnosis of liver fibrosis in rats.Methods Totally 45 SD rats were selected as the experimental group,and liver fibrosis models were established through intraperitoneal injection of 40%CCl 4 olive oil solution,while 12 rats were injected with equal normal saline solution in abdomen and enrolled in control group.MR scans were performed on 5—6 rats in experimental group and 2 rats in control group on the 1st,2nd,4th,6th,8th and 10th week after injection started.The liver of rat was taken out for pathology use,and fibrosis staging was performed after MR scan.Results T1rho values of liver fibrosis for F0—F6 were(40.68±1.60)ms,(42.80±1.08)ms,(44.98±1.04)ms,(46.47±3.47)ms,(47.73±3.84)ms,(55.38±7.46)ms and(57.08±5.11)ms,respectively.The overall difference was statistically significant(F=30.72,P<0.01).T1rho values in liver of rat was positively correlated with liver fibrosis stage(r=0.88,P<0.01),while the expression level ofα-smooth muscle actin was positively correlated with liver fibrosis stages(r=0.74,P<0.01)and T1rho values(r=0.57,P<0.01).Conclusion MR T1rho can be used to quantitatively and noninvasively evaluate the degree of liver fibrosis in rats.