中等强度的全身性稳恒磁场暴露对于2型糖尿病软组织创伤愈合修复的实验研究

Repairing effects of moderate-intensity static magnetic fields on wound healing of type 2 diabetic soft tissue

目的:系统探讨中等强度稳恒磁场(SMF)对于2型糖尿病创伤修复的作用效果,并初步探究其可能的作用机制,为其科学广泛的临床应用奠定基础。方法:于32只3月龄雄性2型糖尿病db/db小鼠及与其同背景的16只野生型小鼠背部构建直径1 cm的圆形创口,并覆盖以透明薄膜伤口敷料。实验分为空白对照组、db/db小鼠组(db/db组)及db/db小鼠施以SMF暴露组(db/db+SMF组),每组16只小鼠。分别于创伤模型构建的第5、12、19天处死每组的4只小鼠。db/db+SMF组小鼠施以峰值4 mT的全身SMF暴露,每天治疗2 h。分别对各组小鼠的创伤闭合率、总愈合时间、创伤组织抗张强度和伤口组织病理形态进行检测,并对白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)和血管内皮生长因子(VEGF)细胞因子表达进行检测分析。结果:SMF于术后的5、12、19 d均显著增加了db/db小鼠的创伤闭合率(P<0.05),降低了创伤总愈合时间(P<0.05),增加了db/db小鼠伤口组织抗张强度(P<0.05),降低了炎性细胞数量并抑制了炎性因子IL-1β、TNF-α和IL-6的表达(P<0.05),但对VEGF的表达影响不显著。结论:中等强度的全身性SMF暴露具有显著的加速2型糖尿病软组织损伤修复的治疗效果,而这一积极效果与其显著的抗炎性反应具有密切关联。

Objective:To explore the effects of moderate-intensity static magnetic fields(SMF)on type 2 diabetic wound healing and its possible mechanism,in order to lay a foundation for its scientific and extensive clinical application.Methods:Round-shape wound of soft tissues with 1-cm diameter was constructed on the dorsum of thirty-two 3-month-old male type 2 diabetic db/db mice and sixteen wild-type mice with the same gene background,followed by being covered with transparent film wound dressing.The experiment was divided into the control group(Control),db/db mice group(db/db),and db/db mice exposed to SMF group(db/db+SMF)with sixteen mice in each group.Four mice in each group were killed post 5,12 and 19 days of the wound model establishment,respectively.The mice in the db/db+SMF group were subjected to systemic SMF exposure(4 mT peak intensity)for 2 h per day.The wound closure rate,overall wound healing period,tensile strength,and histopathological morphology in each group were determined and analyzed.Moreover,the gene expression of Interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α),interleukin-6(IL-6)and vascular endothelial growth factor(VEGF)was measured.Results:SMF significantly increased the wound closure rate and the tensile strength of wound tissues(P<0.05),decreased the overall healing time(P<0.05),and the number of inflammatory cells,as well as inhibited the expression of inflammatory cytokines(IL-1β,TNF-αand IL-6)in db/db mice on day 5,12 and 19 days after surgery(P<0.05),whereas SMF had no significant effect on the VEGF expression.Conclusion:Moderate-intensity systemic SMF exposure exhibited significant therapeutic effects on accelerating wound healing of type 2 diabetic soft tissue,which were closely related to its significant anti-inflammatory response.