IL-8在胃癌组织上皮间质转化过程中表达增强

Enhanced IL-8 expression in epithelial mesenchymal transformation of gastric cancer tissue

目的研究胃癌上皮间质转化(EMT)过程中趋化因子IL-8的调节机制与意义。方法用Western blot、酶联免疫吸附试验(ELISA)等技术,检测胃癌组织与正常组织中IL-8以及EMT相关基因(E-cadherin,vimentin,twist等)的表达,胃癌细胞系MGC-803对IL-8与胃癌细胞EMT的相关性进行分析和探讨。结果肿瘤组织中IL-8的表达水平(710.5±89.9)显著高于癌旁组织(510.5±85.5)(P<0.001)。与正常组织相比,胃癌组织中IL-6、twist及vimentin表达水平明显高于正常胃黏膜组织(P<0.05),而E-cadherin表达水平低于正常组织(P<0.05)。IL-8因子的分泌与twist蛋白(P<0.001)和vimentin蛋白(r=0.454,P<0.001)表达水平呈显著正相关性,与E-cadherin蛋白表达水平呈显著负相关性(P<0.001)。结论胃癌组织中趋化因子IL-8的表达量增强,IL-8与胃癌侵袭转移相关,同时IL-8的表达量也可作为胃癌侵袭转移患者预后判断的参考标准之一。

Objective To investigate the regulatory mechanism and significance of chemokine IL-8 in epithelial mesenchymal transition(EMT) leading to gastric cancer. Methods Western blot, ELISA and other techniques were used to detect the expression of IL-8 and EMT-related genes in gastric cancer tissues and plasma. To study the correlation between IL-8 and EMT in gastric cancer cells with MGC-803. Results The expression level of IL-8 in tumor tissues(710.5±89.9) was significantly higher than that in adjacent tissues(510.5±85.5). Compared to normal tissue,the expression rates of IL-8, twist and vimentin in gastric cancer tissues were significantly higher than those in normal gastric mucosa tissues(P<0.05), while the expression of E-cadherin in gastric cancer tissues were lower than those in normal tissues(P<0.05).The secretion of IL-8 was positively correlated with twist protein(P<0.001), vimentin protein(r=0.454, P< 0.001), and negatively correlated with E-cadherin protein(P< 0.001).Conclusions The expression of chemokine IL-8 is increased in gastric cancer tissues and is correlated with the expression of EMT-related proteins, which indicates that IL-8 is related to the invasion andmetastasis of gastric cancer. Meanwhile, the expression of IL-8 may also be used as one of the criteria in the prognosis of patients, for the invasion and metastasis of gastric cancer.