ELISA方法检测老年人梅毒螺旋体抗体假阳性原因初探

Preliminary Study on The Cause of False Positive Detection of Treponema Pallidum Antibody in Elderly Patients by ELISA

目的:探究ELISA方法检测老年人梅毒螺旋体抗体假阳性的原因。方法:随机选择在2016年10月-2018年10月来我医院接受检查的检测者2000例,将所有检测者分为对照组和老年组,通过ELISA方法检测血清当中的梅毒螺旋体抗体,对于出现阳性检测结果的检测患者来说,再次通过梅毒螺旋体明胶颗粒凝集实验进行进一步的诊断。并将结果进行对比和分析。结果:老年组其阳性率以及假阳性率均高于对照组,并且老年组患者出现假阳性率的概率随着年龄的增长而出现逐渐增高的趋势。结论:对于通过ELISA方法检测患者梅毒螺旋体抗体这一实验来说,老年人出现假阳性率的概率偏高,考虑可能是由于基础疾病、其他内源性干扰因素以及相关螺旋体等因素。

Objective:To investigate the causes of false positive detection of Treponema pallidum antibodies in elderly patients by ELISA.Methods:A total of 2,000 patients who came to our hospital from October 2016 to October 2018 were randomly selected.All the subjects were divided into the control group and the elderly group.The antibodies in the serum were detected by ELISA.For the detection of the test results,the diagnosis was further carried out by the syphilis spirogel gel particle agglutination test.The results were compared and analyzed.Results:The positive rate and false positive rate in the elderly group were higher than those in the control group,and the probability of false positive rate in the elderly group gradually increased with age.Conclusion:For the experiment of detecting treponema pallidum antibody by ELISA,the probability of false positive rate in the elderly is high,considering factors such as underlying diseases,other endogenous interference factors and related spirochetes.