可再生磁共振造影剂介孔二氧化硅纳米粒的制备与表征

Preparation and Characterization of Recycle MR Imaging Functional Mesoporous Silica Nanoparticles

制备一种可再生含钆介孔二氧化硅纳米粒(rMSN-Gd),对其进行表征,探究其应用于核磁共振成像(MRI)的可行性。以稻壳为硅源,掺杂金属钆,制备具有靶向显影功能的rMSN-Gd。透射电镜与扫描电镜观察该纳米材料特征,BET法与X射线衍射表征材料比表面积与介孔状态;能谱分析(EDS)及电感耦合等离子体发射光谱(ICP-AES)检测纳米粒子元素含量;溶血实验表征其生物相容性;磁共振成像系统观察其在体外和BALB/c裸鼠体内Hela移植瘤的显像特征。制备的rMSN-Gd呈圆球形,分散性良好,表面具有规则的介孔结构,平均粒径为150 nm,钆含量(质量分数)为0.47%。材料具有良好生物相容性;体外MRI成像呈浓度梯度性;将该材料静脉注射裸鼠后约30 min,Hela裸鼠移植瘤信号强度明显增高。本研究成功制备了可再生含钆纳米介孔硅rMSN-Gd,其具肿瘤靶向聚集性,可作为新型磁共振造影剂使用。

A regenerative gadolinium-containing mesoporous silica nanoparticles(rMSN-Gd)were prepared and characterized,and its feasibility for magnetic resonance imaging(MRI)was explored.The rice husk is used as a silicon source and gadolinium was incorporated into a recycle mesoporous silica nanoparticle to prepare rMSN-Gd.The characteristics of the nanomaterials were observed by transmission electron microscopy and scanning electron microscopy.The specific surface area and mesoporous state of the materials were characterized by BET and X-ray diffraction.The content of nanoparticles was detected by energy spectrum analysis(EDS)and inductively coupled plasma optical emission spectroscopy(ICP-AES).The hemolysis experiment was used to evaluate its biocompatibility;the MRI system was used to observe the imaging characteristics in vitro and in vivo.The prepared rMSN-Gd was spherical and well dispersed.The globular-shaped rMSN-Gd with Gadolinium content percentage of 4.7%was approximately 150 nm in diameter.These nanoparticles obtain excellent dispersibility and a regular mesoporous structure.The material has good biocompatibility;in vitro MRI imaging showed concentration gradient;about 30 min after intravenous injection of the material into nude mice,the signal intensity of Hela nude mice was significantly increased.A gadolinium-containing nanoparticle rMSN-Gd might be a novel nanoparticle contrast agent for magnetic resonance imaging.