视黄醇结合蛋白在结直肠肿瘤中的差异表达

The Differential Expression of Retinol Binding Protein in Colorectal Tumor

目的研究视黄醇结合蛋白(retinol binding protein,RBP)在结直肠肿瘤切片中的差异表达,探讨视黄醇结合蛋白与结直肠肿瘤恶性度的相关性。方法收集12例正常健康者,12例结直肠息肉患者与47例结直肠恶性肿瘤患者结肠组织切片,利用免疫组织化学检测RBP的差异表达。结果在组织切片中,RBP在正常人与结直肠恶性肿瘤患者,结直肠息肉与恶性肿瘤中的表达差异有统计学意义(P<0.001,P<0.001)。RBP在结直肠恶性肿瘤中呈高表达状态。RBP仅在26%正常组织样本与60%结直肠息肉组织样本中具有阳性表达,在结直肠恶性肿瘤中100%阳性表达。RBP与结直肠肿瘤的分期、分级及是否转移差异无统计学意义(P>0.05)。结论RBP在正常结直肠组织与结直肠恶性肿瘤组织中存在差异表达。

Objective To explore the potential correlation between the expression of retinol binding protein(RBP)and the malignant degree of colorectal tumor.Methods And Methods We collected tissue samples from 12 cases of normal control,12 cases of colorectal polyp patients,and 47 cases of colorectal cancer patients.All the cancer patients were grouped by the clinical characteristics(tumor stage,cancer cell differentiation(grade)and metastases factors).Results Higher retinol binding protein expression was observed in colorectal cancer tissues compared with normal colorectal and colorectal polyp tissues(P<0.001;P<0.001).Positive retinol binding protein staining was detected in 26%of normal control tissues and 60%of colorectal polyp tissues respectively,while in the colorectal cancer tissues,RBP showed 100%positive staining.The expression of retinol binding protein was not associated with the stage of colorectal cancer(P>0.05).Also,retinol binding protein had no relationship with cancer cell differentiation and metastases(P>0.05).Conclusion Retinol binding protein has differential expression in colorectal cancer tissues compared with normal and polyp tissues.