摘要
目的分析生长激素缺乏症(GHD)患儿的Ghrelin的水平及基因多态性与患儿心脏病变的相关性。方法收集65例GHD患儿为GHD组,50例健康体检儿童为对照组。经三维斑点追踪成像超声技术(3D-STI)检测2组的心脏结构和功能指标,包括整体纵行应变(GLS)、整体径向应变(GRS)、整体圆周应变(GCS)、整体扭转角度(GTA)和左室体质量(LVM));ELISA法检测2组血浆的acyl-Ghrelin、desacyl-Ghrelin水平,计算总Ghrelin及acyl-Ghrelin/desacyl-Ghrelin(A/D)值;PCR-RFLP法检测C408A和G346A位点单核苷酸多态性。结果与对照组比较,GHD组的GLS、GRS、GCS、LVM均明显降低(P<0.05或P<0.01),但2组的GTA比较差异无统计学意义(P>0.05);GHD组的acyl-Ghrelin、desacyl-Ghrelin、总Ghrelin水平及A/D值均高于对照组(均P<0.01);GHD组的acyl-Ghrelin、desacyl-Ghrelin水平、总Ghrelin水平与GLS、GRS、GCS均呈负相关(r=-0.548,P=0.001;r=-0.481,P=0.003;r=-0.411,P=0.014;r=-0.520,P=0.001;r=-0.444,P=0.007;r=-0.381,P=0.024;r=-0.536,P=0.001;r=-0.464,P=0.005;r=-0.397,P=0.018),而与GTA、LVM均无相关性(P>0.05);A/D值与GLS、GRS、GCS、GTA、LVM均无相关性(P>0.05);与对照组比较,GHD组GG基因型分布较对照组高(χ2=8.851,P=0.003),GT基因型分布较对照组低(χ2=11.523,P=0.001),CC、CT、TT比较差异均无统计学意义(均P>0.05);GHD组不同基因型对应的GLS、GRS、GCS、GTA、LVM检测值比较差异均无统计学意义(均P>0.05)。结论GHD患儿存在心脏的结构和功能改变,且acyl-Ghrelin、desacyl-Ghrelin、总Ghrelin的水平与心脏的功能改变相关;Ghrelin的C408A基因多态性与GHD的发病相关。
Objective To analyze the relationships of ghrelin levels and gene polymorphisms to cardiac abnormalities in children with growth hormone deficiency(GHD).Methods Cardiac structure and function were detected by three-dimensional cardiac ultrasound speckle tracking imaging(3D-STI)in 65 GHD children and 50 healthy controls,including global longitudinal strain(GLS),global circumferential strain(GCS),global radial strain(GRS)global torsion angle(GTA)and left ventricular mass(LVM).Plasma levels of acyl-ghrelin and desacyl-ghrelin were measured by ELISA to calculate the total ghrelin levels and acyl-ghrelin and desacyl-ghrelin(A/D)ratio.The single nucleotide polymorphisms at C408A and G346A loci were determined by PCR-RFLP.Results Compared with control group,the values of GLS,GRS,GCS and LVM decreased,but the levels of acyl-ghrelin,desacyl-ghrelin,total ghrelin and A/D ratio increased in GHD group(P<0.05 or P<0.01).There was no significant difference in the GTA between the two groups(P>0.05).In GHD group,the levels of acyl-ghrelin,desacyl-ghrelin and total ghrelin were negatively related to the values of GLS,GRS and GCS(r=-0.548,P=0.001;r=-0.481,P=0.003;r=-0.411,P=0.014;r=-0.520,P=0.001;r=-0.444,P=0.007;r=-0.381,P=0.024;r=-0.536,P=0.001;r=-0.464,P=0.005;r=-0.397,P=0.018;respectively),but not correlated with the values of GTA and LVM(P>0.05).Furthermore,the ratio of A/D was not associated with the values of GLS,GRS,GCS,GTA and LVM(P>0.05).The distribution of GG genotype in GHD group was higher than that in control group(χ2=8.851,P=0.003).However,the distribution of GT genotype in GHD group was lower than that in control group(χ2=11.523,P=0.001).No statistical differences were found in the distribution of CC,CT and TT genotypes between the two groups(P>0.05).In GHD group,there were no statistical differences in the values of GLS,GRS,GCS,GTA and LVM among different genotypes(P>0.05).Conclusion There are obvious changes in cardiac structure and function in children with GHD.The levels of acyl-ghrelin,desacyl-ghrelin and total ghrelin are correlated with cardiac function,and C408A polymorphism of ghrelin is related to the onset of GHD.
作者
刘洋
刘燕玲
李国林
章春泉
叶春风
徐丹
胡娜
吴英
许鑫悦
LIU Yang;LIU Yan-ling;LI Guo-lin;ZHANG Chun-quan;YE Chun-feng;XU Dan;HU Na;WU Ying;XU Xin-yue(Department of Pediatrics,the Second Affiliated Hospital of Nanchang University,Nanchang 330006,China;Department of Ultrasonography,the Second Affiliated Hospital of Nanchang University,Nanchang 330006,China)
出处
《南昌大学学报(医学版)》
CAS
2019年第4期35-39,64,共6页
Journal of Nanchang University:Medical Sciences
基金
江西省自然科学基金(20171BAB205042)
金磊儿科内分泌中青年医师成长科研基金(PEGRF201607011)
关键词
生长激素缺乏症
心脏病变
Ghrelin
基因多态性
酰基化
三维斑点追踪成像
growth hormone deficiency
cardiac abnormalities
ghrelin
gene polymorphism
acyl-ghrelin
three-dimensional cardiac ultrasound speckle tracking imaging