黑龙江省大豆推广品种农艺品质性状优异等位变异发掘

Mining of novel alleles of agronomic traits and quality traits in soybean commercial varieties in Heilongjiang Province

发掘推广品种的农艺和品质性状优异等位变异和载体材料可为培育优质大豆品种提供遗传信息和育种资源。本研究以200份黑龙江省近年大豆推广品种为试验材料,利用187对SSR标记进行全基因组扫描,分析群体结构,采用TASSEL软件的GLM方法对标记与3个农艺性状、蛋白质含量及油分含量进行关联分析。结果表明:10个SSR标记与百粒重关联,其中表型效应值最大的优异等位变异为Sat-149-192,载体材料为东农57;8个SSR标记与株高关联,表型效应值最大的等位变异是Satt413-206,载体材料为赤豆1号;13个SSR标记与生育期关联,表型效应值最大的等位变异是Satt631-180,载体材料为黑农61;5个SSR标记与油分含量关联,表型效应值最大的等位变异是Satt234-138,载体材料为合丰55;9个SSR标记与蛋白质含量关联,表型效应值最大的等位变异是Satt632-293,其载体材料为东农43。上述等位变异的信息为培育提供了亲本选配和后代等位条带辅助选择的依据。

Mining of novel alleles carrying varieties of agronomic and quality traits of Heilongjiang cultivated soybean can provide genetic information and breeding materials for cultivating high-quality soybean varieties.The genotypic data of 187 simple-sequence repeat(SSR)markers on 200 commercial soybean varieties in Heilongjiang Province were used and analyzed for association with three agronomic traits,protein content and oil content under TASSEL GLM(general linear model)program based on the population structure analysis.The results showed that 10 SSR loci were associated with 100-seed weight,Sat-149-192 had the highest positive phenotypic effect,and the loci carrying variety was Dongnong 57.Eight SSR loci were associated with plant height,Satt413-206 had the high est positive phenotypic effect,and the loci carrying variety was Chidou 1.Thirteen SSR loci were associated with growth period,Satt631-180 had the highest positive phenotypic effect,and the loci carrying variety was Heinong 61.Five SSR loci were associated with oil content,Satt234-138 had the highest positive phenotypic effect,and the loci carrying variety was Hefeng 55;Nine SSR loci were associated with protein content,Satt632-293 had the high est positive phenotypic effect,and the loci carrying variety was Dongnong 43.The information of multiple alleles of QTL on entire genome could be used in cross design for pyramiding novel alleles and marker-assisted selection in breeding for soybean.