肝癌患者肿瘤组织和血清lncRNAs水平变化的研究

Research on changes of lncRNAs levels in tumor tissues and serum in patients with hepatocellular carcinoma

目的研究肝细胞肝癌(HCC)患者组织和血清中lncRNAs(lncRNA HOTAIR,IncRNA DANCR,IncRNA HOTTIP,IncRNA TCF7)的表达水平。方法采用实时定量PCR方法检测28例肝细胞肝癌(HCC)患者(HCC组)及12例肝血管瘤患者(对照组)组织和血清中IncRNAs HOTAIR、HOTTIP、DANCR和TCF7的表达水平。结果HCC癌组织中IncRNA DANCR和IncRNA TCF7相对表达量明显高于肝血管瘤组织和癌旁组织(P<0.05),IncRNA HOTAIR和IncRNA HOTTIP相对表达量则无明显差异(P〉0.05)。与对照组比较,HCC组血清中IncRNA DANCR和IncRNA HOTTIP表达量显著上调,而IncRNA HOTAIR和IncRNA TCF7表达量则无明显差异(P〉0.05)。血清IncRNA DANCR和IncRNA HOTTIP联合检测诊断HCC的AUC最大为0.929(95%CI:0.808-0.996,P<0.01)。结论肝癌组织IncRNA DANCR和IncRNA TCF7表达水平上调;HCC患者血清IncRNA DANCR和IncRNA HOTTIP表达量显著上调。血清IncRNA HOTTIP和IncRNA DANCR可能是潜在的HCC诊断标志物,有望提高HCC的早期诊断率。

Objective To study the expression of IncRNAs(IncRNA HOTAIR,IncRNA DANCR,IncRNA HOTTIP,IncRNA TCF7)in tissues and serums in patients with hepatocellular carcinoma(HCC).Methods The quantitative real-time PCR method was used to determine the expression levels of IncRNAs HOTAIR,HOTTIP,DANCR and TCF7 in tissues and serums of HCC group(n=28 patients with HCC)and the control group(71=12 patients with hepatic hemangioma).Results The relative expression levels of IncRNA DANCR and IncRNA TCF7 in HCC tissues were significantly higher than that of hepatic hemangioma tissues and para-carcinoma tissues(P<0.05),without significant difference in the relative expression levels of IncRNA HOTAIR and IncRNA HOTTIP(P>0.05).The expression of IncRNA DANCR and IncRNA HOTTIP in HCC group was significantly higher than that in the control group,without significant difference in expression of IncRNA HOTAIR and IncRNA TCF7(P>0.05).The maximum AUC of HCC detected by IncRNA DANCR combined with IncRNA HOTTIP was 0.929(95%CI:0.808-0.996,P<0.01).Conclusion The expression levels of IncRNA DANCR and IncRNA TCF7 in HCC tissues are higher;expression levels of IncRNA DANCR and IncRNA HOTTIP in HCC patients are significantly upregulated.The IncRNA HOTTIP and IncRNA DANCR in serum may be potential diagnostic markers of HCC,which are expected to improve the early diagnostic rate of HCC.