甘肃某牦牛场奇异变形杆菌的分离鉴定及小鼠致病性试验

Isolation,Identification and Phylogenetic Analysis of Proteus mirabilis from Yaks

为研究甘肃地区养殖场牦牛死亡原因,对无菌采集的牛肺组织中的分离菌株进行培养观察、革兰染色、16 S rRNA基因序列分析、药敏试验和小鼠致病性试验。结果表明,该菌为革兰阴性杆菌,在TSA培养基上呈迁徙状生长,难以形成单个菌落,在SS培养基上形成白色圆形中间带黑点的不透明菌落,分离菌株16 S rRNA测序结果BLAST比对后,与其参考菌株同源性为99.6%~99.9%,确定为奇异变形杆菌。药敏试验结果表明,该分离菌株对临床上常用的18种药物中11种药物敏感,6种药耐药,为临床治疗提供了科学依据。致病性试验确定该菌分离的小鼠半数致死量为0.73×10 9CFU。

In order to study the cause of yak death in a Gansu breeding farm,the strain was isolated from Yak lung tissue,cultured and identified by Gram staining and 16 S rRNA gene sequence analysis.The antimicrobial susceptibility test and mouse virulence test were conducted.The results showed that the strain was Gram-negative bacillus and migrated on TSA medium.It was difficult to form a single colony.The opaque colony with black spots was formed on SS medium.The results of 16 S rRNA sequencing of the isolate after Blast with reference strains show 99.6%-99.9%homology,and it was identified as Proteus mirabilis.According to the results of antimicrobial susceptibility test,the isolate was sensitive to 11 kinds of antimicrobials commonly used in clinic,and resistant to 6 kinds of drugs.The results provided the basis for clinical medication.Virulence tests confirmed that LD 50 of the isolate to mice was 0.73×10 9 CFU.