烟叶多糖硒化衍生物制备及其体外抗氧化活性研究

Preparation of Selenized Derivatives from Tobacco Leaf Polysaccharides and Its Antioxidant Activity in Vitro

以烤后烟叶为原料,通过超声辅助提取烟叶粗多糖,经过纯化得到精制多糖,并对精制多糖进行硒化衍生。采用红外光谱对衍生物进行了结构表征,测其硒化取代度;并对烟叶多糖和烟叶多糖硒化衍生物进行体外抗氧化实验。结果表明:采用0.30 mol/L、0.40 mol/L NaCl溶液对多糖进行洗脱时,分离出两种烟叶多糖TPP1、TPP2,两种多糖的硒化取代度分别为0.20,0.24,体外抗氧化实验表明,硒化烟叶多糖的抗氧化活性明显高于烟叶多糖,并且随取代度增大,·DPPH、·OH自由基清除率明显增加。

Using flue-cured tobacco leaves as raw material,the crude polysaccharide was extracted by ultrasound-assisted and refined polysaccharides were purified and selenization of them was done for derivatives.The derivatives were characterized by infrared spectroscopy and their degrees of selenization substitution were measured.On the basis of this,the antioxidative activity of tobacco leaf polysaccharide and selenized tobacco leaf polysaccharide were studied in vitro.The results showed that tobacco leaf polysaccharide TPP1 and TPP2 were isolated when the polysaccharide was eluted with 0.30 mol/L and 0.40 mol/L NaCl solution,respectively.The selenization substitution degree of the two polysaccharides was 0.20 and 0.24,respectively.The antioxidant activity of selenized tobacco leaf polysaccharides was significantly greater than that of tobacco leaf polysaccharides in vitro antioxidant experiments,and the greater the degree of substitution was,the more obviously the·DPPH and·OH radical scavenging rate increased.