摘要
旨为研究棒瑚菌子实体幼菇期(CP-1)和成熟期(CP-2)基因表达的差异,探索棒瑚菌在生长发育过程中的关键基因和关键代谢通路。采用RNA-Seq测序技术对棒瑚菌子实体进行测序并进行生物信息学分析。测序结果显示,共获得8.78 G(CP-1)和8.28 G(CP-2)的分析数据(Clean reads)。KOG功能注释和GO富集分析显示新陈代谢过程、细胞和细胞组分以及氧化还原酶活性是棒瑚菌子实体发育过程中的关键基因门类。基因表达水平分析结果显示,多酚氧化酶、核糖体、糖苷水解酶家族等基因为棒瑚菌子实体幼菇期(CP-1)和成熟期(CP-2)的主要高表达基因,在蛋白质和细胞壁合成中起重要作用。差异表达基因分析结果显示氧化磷酸化和三羧酸循环是棒瑚菌子实体幼菇期(CP-1)生长发育的关键代谢途径,且环境含氧量对棒瑚菌的生长发育有重要影响;同时棒瑚菌成熟期(CP-2)调节一碳化合物浓度和应对各种环境胁迫的分子机制发生了改变。KEGG通路富集结果显示,MAPK信号通路是参与调节棒瑚菌子实体生长发育的关键信号通路,在调控菌丝的极性生长、细胞周期和真菌细胞形态转换中起重要作用。
This work aims to study the differential expressed genes(DEGs)in Clavariadelphus pistillaris fruiting bodies between the period of juvenile mushroom(CP-1)and mature mushroom(CP-2)and to explore the key genes and metabolic pathways during the growth and development of C.pistillaris.RNA-Seq sequencing technology was used to sequence the C.pistillaris fruiting bodies,and their bioinformatics was analyzed.The sequencing results demonstrated that a total of 8.78 G(CP-1)and 8.28 G(CP-2)clean reads were obtained.Functional annotations of gene KOG and GO enrichment analysis showed that the metabolic processes,cellular and cellular parts,and oxidoreductase activities were the key gene categories in the development of C.pistillaris fruiting bodies.The analysis of gene expression level revealed that the main high-expression genes in CP-1 and CP-2 were polyphenol oxidase,ribosome,and glycoside hydrolase family,etc,which played an important role in protein and cell wall synthesis.The results of DEGs showed that oxidative phosphorylation and citrate cycle(TCA cycle)were the key metabolic pathways for the growth and development of CP-1,and the environmental oxygen content had an important effect on the growth and development of C.pistillari;meanwhile,the molecular mechanism of regulating the concentration of C1 compound and the response to various environmental stresses at CP-2 changed.KEGG pathway enrichment showed that MAPK was a key signaling pathway involved in regulating the growth and development of C.pistillaris fruiting bodies,and it played an important role in regulating the polar growth of mycelia,cell cycle and the morphological transformation of fungal cells.
作者
唐贤
丁祥
董明明
朱淼
宋志强
侯怡铃
TANG Xian;DING Xiang;DONG Ming-ming;ZHU Miao;SONG Zhi-qiang;HOU Yi-ling(Key Laboratory of Southwest China Wildlife Resources Conservation,the Ministry of Education,College of Life Science,China West Normal University,Nanchong 637009;College of Environmental Science and Engineering,China West Normal University,Nanchong 637009)
出处
《生物技术通报》
CAS
CSCD
北大核心
2019年第10期119-129,共11页
Biotechnology Bulletin
基金
四川省科技厅应用基础重点项目(2018JY0087)
四川省科技厅重点研发项目(2018NZ0055)
四川省教育厅重大培育项目(16CZ0018)
南充市科技局项目(16YFZJ0043)
关键词
棒瑚菌
RNA-Seq测序
差异表达基因
代谢通路
Clavariadelphus pistillaris
RNA-Seq sequence
differentially-expressed gene
metabolic pathway