三个绵羊品种LHX3基因多态性与生长性状的关联分析

Correlation Analysis Between LHX3 Gene Polymorphism and Growth Traits in Three Sheep Breeds

寻找与绵羊生长性状有关的分子标记,以期为绵羊的分子育种提供理论依据。通过琼脂糖凝胶电泳和一代测序技术,在滩羊(TS)、小尾寒羊(STHS)和兰州大尾羊(LFTS)的LHX3基因中检测到了29 bp片段的插入型(AA)、插入/缺失型(AB)以及缺失型(BB)3种基因型,对3个不同品种绵羊的LHX3基因进行遗传多态性分析,并通过一般线性模型分析了3种基因型与3个品种绵羊不同生长性状的相关性。3个品种绵羊LHX3的多态信息含量(PIC)均为0.25<PIC≤0.5,呈中度多态,表明该位点的遗传变异相对较高。LHX3基因型影响3个品种绵羊的一个或多个生长性状,其中LHX3基因型与滩羊的体长(BL)、体重(BW)、胸深(ChD)和管围(CaC)显著相关(P<0.05),且缺失型基因型的生长性状优于插入型基因型和插入/缺失型基因型绵羊;小尾寒羊的缺失型基因型的胸围(ChC)、胸深(ChD)和管围(CaC)显著高于插入型基因型和插入/缺失型基因型绵羊(P<0.01);兰州大尾羊的缺失型基因型的胸围(ChC)、十字部高(HW)、体长(BL)、体重(BW)和管围(CaC)显著高于插入型基因型和插入/缺失型基因型绵羊(P<0.01)。由此可见在绵羊分子育种中,LHX3中29 bp的缺失型可作为绵羊选育的DNA标记位点。

This work aims to provide a theoretical basis for high quality sheep breeding by finding molecular markers related to the sheep growth traits.Agarose gel electrophoresis and first-generation sequencing technology were used to detect the three genotypes of LHX3 genes in Tan sheep(TS),Small-Tailed Han sheep(STHS)and Lanzhou Fat-Tailed sheep(LFTS),which include insertion(AA),insertion/deletion(AB)and deletion(BB)of 29 bp in LHX3 gene.Genetic polymorphism of LHX3 in three different sheep breeds was analyzed and the correlation between LHX3 different genotypes and the growth traits of three different breeds were analyzed by general linear model.The polymorphic information content(PIC)of LHX3 in the different breeds was as 0.25<PIC≤0.5,indicating a high genetic variation of this point.The LHX3 gene affected one or more growth traits in the three different sheep breeds.In Tan Sheep,the body length(BL),body weight(BW),chest depth(ChD)and cannon circumference(CaC)were significantly higher(P<0.05)in LHX3 gene deletion genotype than insertion ACTCCAgenotype and insertion/deletion genotype.In Small-Tailed Han sheep,the chest circumference(ChC),ChD and CaC were significantly higher(P<0.01)in LHX3 gene deletion genotype than the other two genotypes.In Lanzhou Fat-Tailed sheep,the ChC,BL,BW,hip width(HW)and CaC were significantly higher(P<0.01)in LHX3 gene deletion genotype than the other two genotypes.The 29 bp-deletion genotype in LHX3 may be selected as a DNA marker site in sheep molecular breeding.