姜黄素逆转电离辐射诱导的非小细胞肺癌A549细胞上皮间质转化

Curcumin Reverses Ionizing Radiation-induced Epithelial Mesenchymal Transformation of Non-small Cell Lung Cancer A549 Cells

目的探讨姜黄素对电离辐射诱导的非小细胞肺癌A549细胞上皮间质转化的影响。方法用电离辐射诱导A549细胞发生上皮间质转化,将其命名为A549R。CCK8法检测细胞增殖;Western blot检测姜黄素对A549R上皮/间质标志物表达的影响;划痕实验及Transwell实验检测姜黄素对A549R细胞迁移能力的影响。结果A549R细胞经姜黄素处理后,细胞形态由纺锤形转变为椭圆形上皮形态;E-cadherin表达下调,pan-Keratin表达上调,Vimentin、Twist表达显著下调,N-cadherin表达水平无明显变化;划痕愈合面积随姜黄素浓度的升高而显著下降;A549R细胞迁移能力随姜黄素浓度的升高而显著下降。结论姜黄素通过下调E-cadherin、Vimentin、Twist的表达,并上调Keratin的表达,从而逆转电离辐射诱导A549细胞发生上皮间质转化。

Objective To investigate the effects of curcumin on epithelial mesenchymal transition of nonsmall cell lung cancer(NSCLC)A549 cell line induced by ionizing radiation.Methods The epithelial mesenchymal transformation of A549 cells was induced by ionizing radiation and named as A549R cells.The proliferation of A549 cells was detected by CCK8 assay.The expression levels of epithelial and mesenchymal markers were detected by Western blot in A549R cells treated with curcumin.The effects of curcumin on the migration ability of A549R cells was detected by scratch test and Transwell assay.Results After curcumin treatment,the morphology of A549R cells changed from spindle to oval epithelium;the expression level of E-cadherin was down-regulated,while the expression of pan-Keratin was up-regulated;the expression of Vimentin and Twist were significantly down-regulated,while the expression level of N-cadherin was not significantly changed.Scratch healing area of scratches and cell migration ability were decreased significantly with the increased concentration of curcumin in A549R cells.Conclusion Curcumin reverses the epithelial mesenchymal transformation of A549 cells induced by ionizing radiation by down-regulating the expression of E-cadherin,vimentin,Twist and up-regulating the expression of Keratin.