沉默ANLN基因对胃癌细胞侵袭、迁移、增殖和凋亡的影响

Effect of ANLN Gene Silencing on Invasion, Migration, Proliferation and Apoptosis of Gastric Cancer Cells

目的探讨肌动蛋白结合蛋白ANLN在体外对胃癌细胞的增殖、凋亡、迁移和侵袭的影响。方法RT-PCR和Western blot检测胃癌细胞株、胃黏膜细胞株中ANLN的表达情况和验证siRNA对ANLN基因的敲减情况。划痕实验和Transwell检测沉默ANLN基因表达对胃癌细胞迁移、侵袭能力的影响。CCK-8实验、细胞荧光染色和流式细胞术检测沉默ANLN基因表达对胃癌细胞增殖和凋亡的变化。结果胃癌细胞株SGC-7901/MGC-803中的ANLN的mRNA和蛋白表达显著高于正常胃黏膜细胞株GES-1(P<0.001)。siRNA干扰后RT-PCR和Western blot结果证明转染成功,转染组的mRNA和蛋白表达量与对照组差异有统计学意义(P<0.05)。下调ANLN能够显著降低胃癌细胞的迁移、侵袭、增殖和凋亡能力。结论下调ANLN的表达可以显著降低胃癌细胞侵袭、迁移、增殖和凋亡能力。

Objective To investigate the effect of actin binding protein ANLN on the proliferation,apoptosis,migration and invasion of gastric cancer cells in vitro.Methods RT-PCR and Western blot were used to detect the expression of ANLN in gastric cancer cell lines and gastric mucosal cell lines and verify the knockdown of ANLN gene by siRNA.The effect of silenced ANLN gene expression on the migration and invasion abilities of gastric cancer cells was detected by scratch test and Transwell assay.CCK-8 assay,cell fluorescence staining and flow cytometry were used to detect the effects of silent ANLN gene expression on the proliferation and apoptosis of gastric cancer cells.Results The expression of ANLN mRNA and protein in gastric cancer cell line SGC-7901/MGC-803 were significantly higher than those in gastric mucosa cell line GES-1(P<0.001).The transfection was successful,and the mRNA and protein expressions in the transfection group were statistically different from those in the control group(P<0.05).Down-regulating ANLN expression significantly reduced the migration,invasion,proliferation and apoptosis of gastric cancer cells.Conclusion Down-regulating ANLN expression could significantly reduce the infiltration,migration,proliferation and apoptosis of gastric cancer cells.