肥胖儿童肠道梭菌属的变化及意义

Changes and significance of intestinal clostridium in obese children

目的分析7~14岁单纯性肥胖儿童与正常对照之间肠道梭菌属的相似性及多样性差别,探讨儿童单纯性肥胖对肠道优势菌属的影响。方法收集30例7~14岁单纯性肥胖和30例同年龄段健康儿童的粪便样品,提取粪便菌群总DNA,利用梭菌属16sDNA种属特异性引物,进行PCR扩增,通过变性梯度凝胶电泳(DGGE)分离,获得肥胖儿童和健康儿童肠道梭菌属的分子指纹图谱,进行多样性及相似性分析;同时测量肥胖和正常儿童的体质量、身高、BMI、血压、血脂、瘦素、血浆脂联素,探讨肥胖儿童与肠道优势菌属的关系及临床意义。结果肥胖组儿童BMI、SBP、TG、TC、LEP水平高于正常组(均P<0.05),HDL、ADP平均水平低于正常组(均P<0.05),两组年龄、DBP及LDL水平差异无统计学意义(均P>0.05);肥胖组梭菌属DGGE图谱的条带数目和H’指数均低于正常组,聚类分析结果示肥胖组内个体间及与正常组比较组间相似性均降低;梭菌属两组、正常组及肥胖组各组内DGGE图谱中的S及H’指数,均与BMI和LEP呈负相关,与ADP呈正相关。结论人类肠道菌群梭菌属组成的变化与儿童肥胖相关,肥胖儿童肠道梭菌属的多样性及相似性降低;肥胖儿童肠道梭菌属的多样性与BMI、ADP和LEP相关。

Objective To analyze the diversity of clostridium in simple obesity children aged 7-14 and the difference from that of normal individuals,and to explore children's simple obesity affecting intestinal advantage bacterium of the genus.Methods We have collected fecal samples of thirty 7 to 14 years old cases with simple obesity and 30 healthy children’s faeces samples of the age.All bacterial DNAs were extracted from the faeces,and then clostridium sDNA 16 species specific primers were used for PCR amplification and separation by DGGE.The obese children’s and healthy children’s intestinal clostridium molecular fingerprint,diversity,and similarity were analyzed.At the same time,the obesity and normal children's weight,height,BMI,blood pressure,blood lipid,leptin,and plasma adiponectin were measure to investigate the relationship between the obese children with the advantage of intestinal bacteria genera and its clinical significance.Results The obese children's BMI,SBP,TG,TC,and LEP were higher than the normal group’s(all P<0.05);their average HDL and ADP were less than the normal group’s(both P<0.05);there were no statistical differences in age,DBP,and LDL between the two groups(all P>0.05).The obesity group’s stripe number of clostridium DGGE profiles and H'sample index were lower than the normal group’s;the cluster analysis results showed that the similarities between the individuals in the obese group and between the two group were reduced.The S and H'indexes of DGGE profiles in the two clostridium groups,the normal group,and each obesity group were negatively correlated with BMI and LEP,and positively with ADP.Conclusion Human intestinal flora of clostridium changes is related to childhood obesity;the diversity and similarity of obese children's intestinal clostridium species are decreased;the diversity of obesity children's clostridium is associated with BMI,ADP,and LEP.