水稻磷高效基因OsPHR2转化小麦及转基因植株的耐低肥能力分析

Transformation of Phosphorus Efficient Gene OsPHR2 into Wheat and Tolerance of Transgenic Plants to Low Fertility

磷素是植物生长和发育所必需的大量元素之一,为获得具有磷高效利用特性的转基因小麦植株,以郑麦7698等6个小麦品种(系)为受体,采用基因枪介导转化法对OsPHR 2基因进行了遗传转化,对其阳性转基因后代进行遗传分析,并对高、低肥处理条件下转基因T 1植株的产量等性状进行分析。PCR检测结果表明,转OsPHR 2基因T 0阳性植株有180株;遗传分析结果表明,转OsPHR 2基因T 116个株系中有15个株系OsPHR 2基因分离比符合孟德尔单基因遗传分离规律;高、低肥处理结果表明,低肥条件下OsPHR 2基因可显著提高小麦的单株产量,但高肥条件下增产不显著,转OsPHR 2基因郑麦1342、郑麦7698的单株产量在低肥条件下均较野生型对照显著增加,增幅分别为11.06%、9.27%,主要得益于千粒质量和穗粒数的增加。

Phosphorus is one of the major necessary elements for plant growth and development.In order to obtain transgenic wheat plants with high phosphorus utilization efficiency,OsPHR 2(Oryza sativa L.phosphate starvation response 2)gene was transferred into Zhengmai 7698 and other five wheat varieties(lines)by particle bombardment transformation method.Genetic analysis was carried out in the positive transgenic wheat progenies.Furthermore,the yield and other traits of T 1 transgenic plants under high and low fertility treatments were analyzed.PCR results showed that there were 180 positive transgenic wheat plants in T 0 generation.Genetic analysis revealed that the OsPHR 2 gene segregation ratio of 15 lines among 16 lines was in accordance with the law of Mendelian single gene genetic segregation.The results of high and low fertility treatments showed that OsPHR 2 could significantly increase the wheat yield of single plant under low fertility treatment,but the high fertility treatment was not.The yields of single plant of OsPHR 2-overexpressing transgenic Zhengmai 1342 and Zhengmai 7698 significantly increased by 11.06%and 9.27%compared with wild type plants under low fertility treatment,which was mainly due to the increase of 1 000-grain weight and grain number per spike.