粳稻种质资源苗期耐盐相关性状的SSR标记关联分析

Association Analysis between SSR Markers and Salt-tolerant Traits at Seedling Stage in Japonica Rice Germplasm Resource

选用均匀分布于水稻12对染色体的38个SSR标记对160份粳稻种质资源进行检测,结合水稻种质资源苗期耐盐性评价,发掘耐盐相关性状的分子标记关联位点,以期为耐盐水稻品种的选育提供优良的中间材料和技术保障。结果表明,各耐盐相关性状间存在一定的相关性。耐盐级别与相对苗高、相对地上部鲜质量、相对根鲜质量均呈极显著正相关,与相对茎粗呈显著正相关,与叶片伤害百分率、相对根冠比均呈极显著负相关。利用38对SSR引物,共检测到192个等位基因,其中有效等位基因109.60个,平均有效等位基因数为2.88个,Shannon’s信息指数和Nei’s遗传多样性指数平均值分别为1.139 4和0.586 9。通过关联分析,共检测到11个与水稻苗期耐盐相关性状关联的SSR位点,分布于水稻1、3、4、7、8、9、10、11、12染色体上,表型贡献率介于4.10%~18.85%,其中,表型贡献率最高的等位变异是与相对地上部鲜质量相关联的RM286-7,增加耐盐级别表型效应最大的等位变异是RM7585-1,表型贡献率为6.80%,载体种质为Bertone。

In order to provide excellent intermediate materials and technical support for the breeding of salt-tolerant rice varieties,thirty-eight pairs of simple sequence repeat(SSR)markers which were uniformly distributed throughout rice genome were used to detect 160 japonica rice germplasm resources,and the molecular marker sites associated with salt-tolerant traits were explored.The results showed that there was certain correlation among salt-tolerant traits.Salt tolerant score was significantly positively correlated with relative plant height,relative shoot fresh weight,relative root fresh weight and relative stem diameter.Salt tolerant score was significantly negatively correlated with leaf damage percentage and relative ratio of root to shoot.A total of 192 alleles were identified at all of 38 SSR loci,the effective alleles number was 109.60,and the effective alleles number per marker locus was 2.88.The average values of Shannon’s information index and Nei’s genetic diversity index of these SSR markers were 1.139 4 and 0.586 9,respectively.Eleven sites were significantly associated with salt tolerance related traits,which were located on chromosomes 1,3,4,7,8,9,10,11 and 12,and explained the phenotypic variance from 4.10%to 18.85%.RM286-7 that was related with relative shoot fresh weight had the highest phenotypic contribution rate,and RM7585-1 had the highest positive phenotypic effect for salt tolerant score,whose phenotypic contribution rate was 6.80%,and the carrier variety was Bertone.