刺五加多糖镇痛抗炎作用及其免疫调节机制的研究

Study on analgesic and anti-inflammatory effect and immunoregulatory mechanism of polysaccharides in Radix et Caulis Acanthopanacis Senticosi

目的探讨刺五加多糖的镇痛抗炎作用及其免疫调节机制。方法①将60只SPF级昆明种小鼠随机分为正常组、对照组、阳性对照组、刺五加多糖高剂量组(ASPS200)、刺五加多糖中剂量组(ASPS100)、刺五加多糖低剂量组(ASPS50)。除正常组(10只)外,其余50只小鼠采用热板法或醋酸扭体法建立镇痛模型,各组进行相应干预,测定各组小鼠镇痛率、扭体反应抑制率、脾脏指数、胸腺指数及血清中细胞因子IL-6、TNF-α和IL-1β的水平。②将60只SPF级昆明种小鼠随机分为正常组、对照组、阳性对照组、刺五加多糖高剂量组(ASPS200)、刺五加多糖中剂量组(ASPS100)、刺五加多糖低剂量组(ASPS50)。除正常组(10只)外,其余50只小鼠采用鸡蛋清致小鼠足趾肿胀或醋酸致小鼠腹腔毛细血管通透性增加建立炎症模型,各组进行相应干预,测定足趾肿胀抑制率、腹腔毛细血管通透性提高抑制率、脾脏指数、胸腺指数及血清中细胞因子IL-6、TNF-α和IL-1β的水平。结果①与对照组比较,阳性对照组和刺五加多糖高剂量组小鼠痛阈值延长(P<0.05),扭体抑制率提高(P<0.05),小鼠血清中细胞因子IL-6、TNF-α和IL-1β的水平不同程度下降(P<0.05);刺五加多糖各剂量组小鼠脾脏指数和胸腺指数不同程度高于对照组(P<0.05);刺五加多糖各剂量组小鼠脾脏指数、胸腺指数、血清中细胞因子IL-6、TNF-α和IL-1β的水平不同程度高于阳性对照组(P<0.05)。②与对照组比较,阳性对照组和刺五加多糖高剂量组小鼠足趾肿胀抑制率和腹腔毛细血管通透性提高的抑制率升高(P<0.05),小鼠血清中细胞因子IL-6、TNF-α和IL-1β的水平不同程度下降(P<0.05);刺五加多糖各剂量组小鼠脾脏指数和胸腺指数不同程度高于对照组(P<0.05)。刺五加多糖各剂量组小鼠脾脏指数、胸腺指数、血清中细胞因子IL-6、TNF-α和IL-1β的水平不同程度高于阳性对照组(P<0.05)。结论刺五加多糖可改善小鼠镇痛抗炎模型状况,其作用机制可能是改善免疫器官指数,调节相关免疫细胞因子水平。

Objective To explore the analgesic and anti-inflammatory effect and the immunoregulatory mechanism of polysaccharides in Radix et Caulis Acanthopanacis Senticosi. Methods ①Sixty SPF Kunming mice were randomly divided into normal group, control group, positive control group and high-, medium-and low-dose groups of polysaccharides in Radix et Caulis Acanthopanacis Senticosi. Except for the normal group(10 mice), the other 50 mice were used to establish the analgesic model by the hot plate method or the acetic acid writhing method. Corresponding intervention was given to each group. Analgesicrate, writhing response inhibition rate, spleen index, thymus index and serum levels of cytokines IL-6,TNF-alpha and IL-1 bet were measured in each group.(2)Sixty SPF Kunming mice were randomly divided into normal group,control group,positive control group and high-,medium-and low-dose groups of polysaccharides in Radix et Caulis Acanthopanacis Senticosi. Except for the normal group( 10 mice),the other 50 mice were taken to establish the model of inflammation by using egg white to induce toe swelling or using acetic acid to induce increase in peritoneal capillary permeability. Corresponding intervention was given to each group. Inhibition rate of toe swelling,inhibition rate of increase in peritoneal capillary permeability,spleen index,thymus index and serum levels of cytokines IL-6,TNF-α and IL-1β were observed in each group. Results(1)Compared with the control group,there were increase in pain threshold( P < 0. 05) and writhing inhibition rate( P<0.05),and decrease to varying degrees in the levels of serum cytokines IL-6,TNF-α and IL-1β( P<0.05) in the positive control group and the high-dose group,and spleen index and thymus index increased to varying degrees in the high-,medium-and low-dose groups( P<0.05). The spleen index,thymus index and the levels of serum cytokine IL-6,TNF-α and IL-1β to varying degrees in the high-,medium-and low-dose groups were higher than the positive control group( P<0.05).(2) Compared with the control group,there were increase in the inhibition rate of toe swelling and inhibition rate of increase in peritoneal capillary permeability( P < 0. 05),and decrease in the levels of serum cytokines IL-6,TNF-α and IL-1β( P<0.05) in the positive control group and the high-dose group,and spleen index and thymus index increased to varying degrees in the high-,mediumand low-dose groups( P<0.05). The spleen index,thymus index and the levels of serum cytokine IL-6,TNF-α and IL-1β to varying degrees in the high-,medium-and low-dose groups were higher than the positive control group( P < 0. 05). Conclusion Polysaccharides in Radix et Caulis Acanthopanacis Senticosi improve the condition of mice in the analgesic and anti-inflammatory model. The mechanism of action may be improving the index of immune organs and regulating the levels of related immune cytokines.