西兰花茎叶肽的酶法制备及其降血脂功能研究

Enzymatic Preparation of Brassica oleracea L.var.botrytis L Stem and Leaf Peptide and Its Hypolipidemic Function

本文以西兰花茎叶为原料,用煮沸法从中提取蛋白。蛋白粗提物经酶解制备,得到西兰花肽后用Valflow50超滤、Sephadex G-15分离纯化,通过细胞实验进行降血脂功能筛选,最后通过动物实验对其降血脂功能进行评价。结果表明,西兰花蛋白粗提物的蛋白质含量为49.6%,酶解可以增强西兰花蛋白的降血脂活性,分离组分G15-Ⅱ的体外降血脂功能最强。以400、200 mg/kg灌胃的大鼠血液总胆固醇(TC)和甘油三酯(TG)含量水平降低(P<0.01),同时400 mg/kg组的HDL-C含量提高(P<0.05),表明G15-Ⅱ具有降血脂作用。此研究可为西兰花的深度开发和综合利用提供基础数据和科学参考。

In this paper,broccoli stems and leaves were used as raw materials,and proteins were extracted by boiling.The crude protein extracts were prepared by enzymatic hydrolysis to obtain the broccoli peptide extracts,which was then separated and purified by Valflow50 ultrafiltration and Sephadex G-15.The blood lipid-lowering function was screened by cell experiments.Finally,the blood lipid-lowering function was evaluated by animal experiments.The results showed that the protein contents of broccoli protein crude extracts was 49.6%.Enzymatic hydrolysis could enhance the hypolipidemic activity of broccoli protein,and the isolated component G15-Ⅱhad the strongest antihyperlipidemic function in vitro.The levels of TC and TG in blood of rats treated with 400 and 200 mg/kg were decreased(P<0.01),and the levels of HDL-C in 400 mg/kg group were increased(P<0.05),which indicated G15-Ⅱhad a blood lipid lowering effect.This study would provide basic data and scientific references for the in-depth development and comprehensive utilization of broccoli.