菊粉复配灵芝多糖对2型糖尿病大鼠的降血糖作用

Hypoglycemic Effect of Inulin Combined with Ganoderma lucidum Polysaccharides on Type 2 Diabetes Mellitus Rats

为探究菊粉复配灵芝多糖降血糖作用,采用高糖高脂联合链脲佐菌素(STZ)诱导SD大鼠建立2型糖尿病模型,记录菊粉复配灵芝多糖干预期间大鼠每周体重(BW)和血糖(FBG)变化,5周后检测其血清中胰岛素(FINS)、糖化血清蛋白(GSP)、谷丙转氨酶(ALT)和谷草转氨酶(AST)及肝糖原、肌糖原等指标,并进行肝脏的HE染色观察。结果表明,和模型组相比,菊粉复配灵芝多糖高剂量组(5 g/kg·bw菊粉+0.4 g/kg·bw灵芝多糖)大鼠的FBG、FINS、GSP、ALT和AST等指标极显著降低(P<0.01),糖原含量极显著增加(P<0.01),且和单一菊粉组相比存在显著差异(P<0.05);HE染色观察发现菊粉复配灵芝多糖可有效缓解2型糖尿病大鼠的肝脏损伤。综上所述,菊粉复配灵芝多糖对2型糖尿病大鼠有明显的降血糖作用,该作用机制可能与改善机体胰岛素抵抗、修复肝脏损伤和促进糖原合成有关。

To investigate the hypoglycemic effect of the inulin compound with Ganoderma polysaccharides,type 2 diabetes mellitus rat model induced by high fat diet and streptozotocin was established.Then the inulin and Ganoderma lucidum polysaccharides were administered orally to the diabetic rats for 5 weeks.During this period,the body weight and fasting blood glucose of T2DM rats(FBG)were measured weekly.Subsequently,the insulin(FINS),glycosylated serum protein(GSP),glutamic acid transaminase(ALT),aspartate aminotransferase(AST),hepatic glycogen and muscle glycogen were also measured,and the HE staining of the liver was observed.The results showed that,compared with the model group,the indicators of FBG,FINS,GSP,ALT and AST of the high combination of inulin and Ganoderma lucidum polysaccharides groups(5 g/kg·bw inulin+0.4 g/kg·bw Ganoderma lucidum polysaccharides)were significantly decreased(P<0.01),the contents of glycogen was significantly increased(P<0.01),and insulin resistance was significantly improved(P<0.01).Moreover,compared with the simple inulin group,all the above indexes in the compound groups were significantly different(P<0.05).In a conclusion,inulin combined with Ganoderma lucidum polysaccharides could enhance synergistic hypoglycemic effect by improving insulin resistance,repairing liver injury and promoting glycogen synthesis.