基于主成分分析的刺葡萄实生单株苗期性状综合评价

Characterization of Seed-derived Vitis davidii Seedings by Principal Component Analysis

【目的】建立一套适合刺葡萄实生单株苗期性状的评价方法。【方法】测定了刺葡萄实生单株干径、新梢长度、叶片叶绿素含量、叶面积、叶片干鲜重、叶片叶形结构参数等16个主要性状,并应用主成分分析法对其进行综合评价。【结果】刺葡萄实生单株主要生长性状均出现较大变异,尤其是新梢长度、叶片叶绿素含量、干径等变异较大。选取方差累积贡献率为86.254%的前6个主成分来评价刺葡萄实生单株,决定第1主成分的是干径、新梢长度、叶绿素含量、叶面积、叶片鲜重、叶片干重;决定第2主成分的是中脉长、上侧脉长、下侧脉长、叶柄脉长;决定第3主成分的是叶宽和上中侧脉夹角。【结论】以6个主成分及单个主成分所对应的特征值占所提取主成分总的特征值之和的比例作为权重,构建刺葡萄实生单株苗期性状的综合评价模型F综=0.38F 1+0.24F 2+0.12F 3+0.10F 4+0.09F 5+0.06F 6,综合得分排名前两位的单株分别为S4和S35。

【Objective】To establish a method for evaluating the characteristics of seed-derived Vitis davidii seedlings.【Method】Sixteen characteristics including stem girth,shoot length,leaf chlorophyll content,leaf area,leaf dry and fresh weight,and blade structure of the seedlings were determined for a comprehensive evaluation by principal component analysis.【Result】Significant variations were found on the growth of the seedlings,especially on shoot length,leaf chlorophyll content,and stem girth.The first 6 principal components made up 86.254%of the total contribution.The first principal component was determined by the stem girth,shoot length,chlorophyll content,leaf area,and fresh and dry weights of the leaves;the second principal component by the lengths of midrib,upper lateral vein,lower lateral vein,and downmost vein;and,the third principal component by the leaf width and the angle between upper lateral vein and midrib.【Conclusion】Using the eigenvalues corresponding to the 6 principal components and the ratio of single to extracted principal component,a comprehensive evaluation model for the seed-derived V.davidii seedlings was established.Accordingly,S4 and S35 scored the highest among the specimens.A rapid method to screen V.davidii resources at seedling stage for cultivar selection and new variety breeding had thus become available.