双孢蘑菇6个菌株不同发育阶段的转录组分析

Transcriptome Sequencing on Six Agaricus bisporus Strains at Four Developmental Stages

【目的】通过多个双孢蘑菇菌株不同发育阶段的差异转录组分析,为进一步验证双孢蘑菇发育相关基因及探讨其分子机理奠定基础。【方法】对双孢蘑菇主栽品种As2796及其亲本02、8213,其回交子代W192,以及国外野生菌株ARP159、国内野生菌株AgLH830共6个具有重要代表性的菌株子实体原基期、幼菇期、采摘期、开伞期等4个不同发育阶段共24个样品进行转录组测序,并与双孢蘑菇参考基因组序列进行比对,根据比对结果进行各基因在不同样品中的表达量分析及差异表达基因识别,发掘新基因与共同基因的差异表达,并进行各数据库的基因功能注释。【结果】结果共鉴定到10 660个转录本,发掘新基因677个,其中237个得到功能注释。与原基期相比,6个菌株在幼菇期、采摘期和开伞期分别有49、82、73个共同差异表达基因,其中有13个是相同的基因。【结论】发现了一批在双孢蘑菇子实体不同发育阶段具有显著差异表达的基因,筛选出不同菌株不同阶段的共有差异基因,对双孢蘑菇子实体发育中重要的差异基因进行了注释与探讨。

【Objective】Transcriptome analysis was conducted on Agaricus bisporus at 4 developmental stages to identify the genes associated with and decipher the molecular mechanism involving the fungal development.【Method】From 6 representative strains of A.bisporus including main cultivar As2796,its parents 02 and 8213,its backcrossing offspring W192,foreign wild strain ARP159,and domestic wild strain AgLH830,at primordium,young,harvesting,and opening stages,24 fruiting body specimens were collected for transcriptome sequence analysis.By aligning them against the reference genome sequence of A.bisporus,the genes that were differentially expressed were identified.Both unique and common differentially expressed genes(DEGs)were clearly exposed to be annotated using the databases to determine their specific functions.【Result】Among the 10 660 transcripts obtained,677 genes were unique with 237 functionally annotated.The tested A.bisporus shared 49 common DEGs between primordium and young stages,82 between primordium and harvest stages,and 73 between primordium and pileus opening stages.And,13 genes were found commonly present in the various strains.【Conclusion】Both unique and common DEGs in A.bisporus at the 4 developmental stages were identified and annotated in the study.