期刊文献+

Bt水稻对褐飞虱若虫肠道可培养细菌组成的影响 被引量:1

Effects of Bt Rice on the Structure of Culturable Bacteria in the Gut of Nilaparvata lugens Nymphae
下载PDF
导出
摘要 【目的】探究Bt水稻对褐飞虱若虫肠道可培养细菌组成结构的影响,为转基因水稻的安全性评价提供科学依据。【方法】利用传统微生物纯培养法分别从取食非转基因(CK)水稻和转crylAb抗虫(Bt)水稻的褐飞虱肠道内分离细菌,利用16s rDNA对分离菌株进行鉴定,比较分析转基因水稻对褐飞虱若虫肠道微生物群落结构的影响。【结果】研究结果显示在取食CK水稻和Bt水稻的褐飞虱肠道内各分离获得7株细菌。取食CK水稻的褐飞虱肠道细菌分别属于3个门5个属;而取食Bt水稻的褐飞虱肠道细菌分别属于2个门4个属;分离获得频率最高的是变形菌门细菌Proteobateria,其中取食两种水稻均能分离获得变形菌门的泛菌属Pantoea,以及厚壁菌门Firmicutes的葡萄球菌属Staphylococcus等2个属的细菌。取食CK水稻的褐飞虱肠道内分离获得变形菌门的草螺菌属Herbaspirillum和不动杆菌属Acinetobacter,以及放线菌门的微杆菌属Microbacterium等3个特异性细菌属;取食Bt水稻的褐飞虱肠道内分离得到变形菌门的肠杆菌属Enterobacter和伯克氏菌属Burkholderia等2个特异性细菌属。【结论】取食非转基因水稻和转基因水稻的褐飞虱肠道可培养细菌组成存在一定的差异。本研究为后续深入研究褐飞虱肠道共生细菌的功能,以及转基因水稻对非靶标生物肠道细菌的影响提供了材料和基础。 【Objective】The present study aims to explore the effects of Bt rice on gut bacterial structure of the Nilaparvata lugens nymphae and to provide evidence for rational safety assessment of Bt rice.【Method】Gut bacteria from BPH feeding on non-transgenic rice(CK)and transgenic rice(Bt)were isolated using traditional culture methods,and were identified using 16s rDNA.The composition of isolates was compared between these two treatments.【Results】The results showed that seven bacterial strains were isolated from the gut of N.lugens feeding on either CK or Bt rice.Three phyla and five genera of the gut bacteria were identified from N.lugens feeding on CK rice,while two phyla and four genera of the gut bacteria from N.lugens feeding on Bt rice.The taxa of bacteria identified from the guts of N.lugens either feeding on CK rice or Bt rice were Proteobateria(including the genus of Pantoea)and Firmicutes(including the genus of Staphylococcus).Herbaspirillum Acinetobacter and Microbacterium were specific genera from the CK treatment,while Enterobacter,and Burkholderia from the Bt treatment.【Conclusion】The composition of culturable bacteria in the N.lugens gut was different beween the CK treatment and the Bt treatment.The findings provide an important foundation for further studies on the function of symbiotic bacteria in N.lugens,and the effects of transgenic rice on the gut bacteria of non-target species.
作者 林胜 李强 夏晓峰 尤民生 LIN Sheng;LI Qiang;XIA Xiao-feng;YOU Min-sheng(State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Agriculture and Forestry University,Fuzhou,Fujian350002,China;Institute of Applied Ecology,Fujian Agriculture and Forestry University,Fuzhou,Fujian350002,China;Joint International Research Laboratory of Ecological Pest Control,Ministry of Education,Fuzhou,Fujian350002,China;Key Laboratory of Integrated Pest Management of Fujian and Taiwan,China Ministry of Agriculture,Fuzhou,Fujian350002,China)
出处 《福建农业学报》 CAS CSCD 北大核心 2019年第7期802-809,共8页 Fujian Journal of Agricultural Sciences
基金 福建省自然科学基金项目(2017J01432) 国家自然科学基金项目(31870520) 福建农林大学科技创新专项基金(CXZX2017317) 国家转基因生物新品种培育重大专项课题(2016ZX08011-001)
关键词 褐飞虱 转基因水稻 肠道细菌 非靶标效应 生物安全 Nilaparvata lugens genetically modified rice gut bacteria non-target effect biosafety
  • 相关文献

参考文献9

二级参考文献194

共引文献195

同被引文献17

引证文献1

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部