饲喂幼虫粪便提取物对马铃薯块茎蛾生殖的影响

Effect of Feeding Extract of Larval Frass on Reproduction of Phthorimaea operculella

将高密度(45头/130 g块茎)饲养幼虫的粪便用清水提取后获得粗提物,在粗提物中添加适量蜂蜜后供马铃薯块茎蛾成虫(正常密度15头/130 g)取食,观察成虫生殖情况。结果表明:喂食幼虫粪便提取物后,能够引发雌虫孤雌现象产生。未交配雌虫取食幼虫粪便提取物后,所产卵粒中正常卵粒的比例显著增加,但单雌产卵量无显著变化。成虫取食幼虫粪便提取物后再进行交配,则可引起卵孵化率的显著下降。

The larva were fed at high density(45 larvae per 130 gram of tuber)of potato tubers,the distilled water extract of fresh frass of larvae were prepared,and honey was then added into the extract to achieve the extract-solution.The adults from larvae reared at normal density(15 larvae/130 gram of tubers)were fed with the extract-solution,and then their reproduction and egg deposition were observed.The results showed that parthenogenesis occurred when they were fed with the extract solution.Feeding the solution could stimulate virgin females to deposit more fully-developed eggs though total eggs did not changed.Furthermore,egg hatch decreased significantly when the adults were fed with the solution and then mated.