miR-92a-3p对TNF-α,IL-6和DcR3的影响

Effects of miRNA-92a-3p on TNF-α,IL-6 and DcR3

目的探讨miR-92a-3p对肿瘤坏死因子-α(TNF-α),白细胞介素-6(IL-6)和诱导因子3(DcR3)的影响。方法用人急性单核细胞白血病细胞(THP-1)设置阴性对照组(NC)。用1μg/mL LPS分别处理THP-1细胞6 h和24 h,实时荧光定量聚合酶链反应(q-PCR)检测TNF-α,IL-6和DcR3的表达,作为Sepsis组(NC+LPS)。利用1μg/mL的LPS处理过表达miR-92a-3p的THP-1细胞,作为过表达miR-92a-3p的Sepsis组(miR-92a-3p+LPS)。利用q-PCR,酶联免疫吸附试验(ELISA)和Western blot分别检测各组TNF-α,IL-6和DcR3的表达情况。结果q-PCR,ELISA和Western blot检测结果显示,TNF-α和IL-6在LPS组、miR-92a-3p+LPS组和NC组的相对表达量均呈下调趋势,差异有统计学意义(P<0.05),DcR3表达差异无统计学意义(P>0.05)。结论miR-92a-3p可抑制TNF-α和IL-6的释放,具有抗炎作用,但是其对DcR3的作用不显著。

Objective To investigate the effects of miRNA-92a-3p on tumor necrosis factor-alpha(TNF-α),interleukin-6(IL-6)and decoy factor-3(DcR3).Methods Human acute mononuclear leukemia cells(THP-1)were used as negative control group(NC).THP-1 cells were treated with 1μg/mL LPS for 6 h and 24 h,respectively.The expression of TNF-a,IL-6 and DcR3 was detected by q-PCR as Sepsis group(NC+LPS).THP-1 cells overexpressing miRNA-92a-3p were treated with 1μg/mL LPS as sepsis group overexpressing miRNA-92a-3p(miRNA-92a-3p+LPS).The expression of TNF-α,IL-6 and DcR3 was detected by q-PCR,ELISA and Western blot.Results The results of q-PCR,ELISA and Western blot showed that the relative expression of TNF-αand IL-6 in LPS group,Mi-92a-3p+LPS group and NC group decreased(P<0.05),while the expression of DcR3 had no statistical significance(P>0.05).Conclusion MiR-92a-3p can inhibit the release of TNF-αand IL-6,and has anti-inflammatory effect,but its effect on DcR3 is not significant.