益气化痰通络方对急性脑缺血模型大鼠缺血海马区神经再生及脑内脑源性神经营养因子表达的影响

Effects of Yiqi Huatan Tongluo Decoction on Nerve Regeneration and Expression of Brain-derived Neurotrophic Factor (BNDF) in Hippocampus of Rats with Acute Cerebral Ischemia (ACI)

目的观察益气化痰通络方对急性脑缺血模型大鼠缺血海马区神经再生及脑内脑源性神经营养因子(BDNF)表达的影响。方法将40只雄性SD大鼠随机分为4组,每组10只,分别为假手术组、模型组、尼莫地平组和益气化痰通络方组,采用改良线栓法制备急性脑缺血大鼠模型。Ayelet Levy评分法测评大鼠神经功能评分,观察大鼠缺血海马区神经再生情况以及脑内BDNF表达的变化。结果模型组大鼠的海马神经元密度和神经功能缺损评分、Caspase-3蛋白含量明显下降(P <0.05),而海马神经元细胞脱落率、Bcl-2蛋白含量、BrdU阳性细胞数目以及BDNF mRNA、BDNF阳性表达和BDNF蛋白明显上升(P <0.05);给予尼莫地平和益气化痰通络方治疗后大鼠海马神经元密度和神经功能缺损评分、Caspase-3蛋白含量以及BDNF m RNA、BDNF阳性表达和BDNF蛋白明显提升(P <0.05),海马神经元细胞脱落率、Bcl-2蛋白含量明显下降(P <0.05),且尼莫地平组与益气化痰通络方组神经功能评分、细胞脱落率、BrdU阳性细胞数目以及BDNF m RNA和BDNF阳性表达比较,差异有统计学意义(P <0.05)。结论益气化痰通络方可改善急性脑缺血模型大鼠缺血海马区神经再生和神经功能损伤,提高BDNF表达。

Objective:To investigate the effects of Yiqi Huatan Tongluo Decoction on nerve regeneration and expression of BNDF in the hippocampus of ACI rats.Methods:40 SD rats were randomly divided into four groups,10 in each:sham operation group,the model group,nimodipine group and Yiqi Huatan Tongluo Decoction group.The modified suture method was used for ACI model.The neurological function scores were measured by Ayelet Levy score,and the neuronal regeneration in the hippocampus and the expression of BNDF in the brain were ob served.Results:The density of hippocampal neurons,the score of neurological deficit and the content of Caspase-3 protein in the model group decreased significantly(P<0.05);the exfoliation rate of hippocampal neurons,the content of Bcl-2 protein,the number of BrdU positive cells,BDNF mRNA,BDNF positive expression and BDNF protein were significantly increased(P<0.05).After treatment with Nimodipine and Yiqi Huatan Tongluo Decoction,the density of hippocampal neurons and neurological deficit score,Caspase-3 protein content,BDNF mRNA,BDNF positive expression and BDNF protein in rats were significantly increased(P<0.05).The percentage of neuronal exfoliation and the content of Bcl-2 protein in hippocampus decreased significantly(P<0.05).There were significant differences in neurological function score,cell exfoliation rate,the number of BrdU positive cells,BDNF mRNA and BDNF positive expression between Nimodipine group and Yiqi Huatan Tongluo Decoction group(P<0.05).Conclusion:Yiqi Huatan Tongluo Decoction can improve the nerve regeneration and neurological damage in the ischemic hippocampus of ACI rats and improve the expression of BDNF.