用荧光图像示踪不同分化的食管癌细胞Cx43的空间分布

Tracking the Spatial Distribution of Cx43 in Different Differentiated Esophageal Cancer Cells Using Fluorescence Images

研究不同分化的食管癌细胞Cx43蛋白的表达和空间定位及其对食管癌细胞的生物学行为的影响。应用合适的荧光探针和激光扫描共聚焦显微镜,通过单粒子跟踪图像分析,检测Cx43蛋白荧光团在食管癌细胞中的表达和分布。Cx43在高分化食管鳞癌EC9706中的分布与EC109细胞相似,特别是细胞膜呈现明显的荧光团;近细胞膜Cx43蛋白含量相对细胞质增高,具有在细胞膜构建细胞通信的结构基础,预示着细胞向良性发展的趋势。低分化食管鳞癌KY150细胞和食管癌SHEEC细胞中Cx43蛋白的荧光团主要分布在近核膜的细胞质中,较少分布在细胞膜附近;近核膜的Cx43蛋白含量比近细胞膜的含量高,说明Cx43蛋白在细胞质中的滞留状态。研究显示SPT图像可以直观地示踪Cx43蛋白荧光团在食管癌细胞中的分布,从而确定Cx43蛋白在不同分化的食管癌细胞中表达及定位的痕迹。

To study the expression and spatial localization of Cx43 protein in different differentiated esophageal cancer cells and its effect on the biological behavior of esophageal cancer cells.The expression and distribution of Cx43 protein fluorophores in esophageal cancer cells were detected by image analysis of single particle tracking(SPT)with appropriate fluorescent probe and laser scanning confocal microscope.The distribution of Cx43 in EC9706 cells of highly differentiated esophageal squamous cell carcinoma was similar to that in esophageal carcinoma EC109 cells,especially the cell membrane showed obvious fluorophores.The content of Cx43 protein in the near-cell membrane was higher than that in the cytoplasm,which had the structural basis for building cell-cell communication of the cytomembrane and indicated the trend of benign development of the cell.The fluorescences of Cx43 protein in KY150 cells of poorly differentiated esophageal squamous cell carcinoma and SHEEC cells of esophageal carcinoma were mainly distributed in the cytoplasm near the nuclear membrane,but less distributed near the cell membrane.The content of Cx43 protein in the near-nuclear membrane was higher than that in the near-nuclear membrane,indicating the retention of Cx43 protein in the cytoplasm.Studies have suggested that SPT images can visually trace the distribution of fluorophore of Cx43 protein in esophageal cancer cells,so as to determine the trace of expression and localization of Cx43 protein in different differentiated esophageal cancer cells.