QCM实时监测五价砷胁迫对水稻悬浮细胞粘弹性的影响

Real-time Monitoring of the Effect on the Viscoelasticity of Rice Suspension Cells with Arsenic (V) Stress Using QCM

本文通过QCM技术实时监测水稻悬浮细胞在不同浓度五价砷连续及单独胁迫下的粘弹性变化,从细胞整体水平评估了重金属对水稻悬浮细胞力学性质的影响。结果表明:1)在2~10 mmol/L范围内,五价砷对水稻悬浮细胞的连续和单独胁迫的影响均随着浓度增加而增强;2)在连续胁迫和单独胁迫下,随着五价砷浓度的增加,水稻悬浮细胞粘弹性指数均下降,细胞软硬度减小即细胞变软;3)在五价砷连续梯度浓度胁迫下,水稻悬浮细胞粘弹性指数呈梯度下降;4)在五价砷不同浓度单独胁迫下,随着五价砷浓度的增加,水稻悬浮细胞粘弹性指数变化逐渐平缓,即水稻悬浮细胞随着五价砷浓度升高,敏感程度降低。以期建立一种用来衡量重金属胁迫对植物影响的一种新方法。

In this study,the effects of cell viscoelasticity under continuous and individual stress from different As(V)concentrations for the rice suspension cells was monitored in real-time by QCM and it was estimated on a overall level of cells.The results shows that:1)In the range of 2~10 mmol/L,the effects of cell viscoelasticity under continuous and individual stress from As(V)increased with increasing concentrations of As(V);2)Under continuous and individual stress,the viscoelasticity and hardness of rice suspension cells declined with the increasing concentrations of As(V);3)The viscoelasticity index of rice cells declined under the stress of continuous gradient concentrations of As(V);4)The sensitivity of rice suspension cells declined with the increasing concentrations of As(V).In the goal of establishing a new method to evaluate the effects of stresses of HMs on plants.