木芙蓉叶提取物体外抗RSV作用机制研究

Study on the anti-RSV mechanism of extracts from Hibiscus mutabilis leaf in vitro

目的:研究木芙蓉叶提取物的体外抗呼吸道合胞病毒(respiratory syncytial viruses,RSV)作用机制。方法:以利巴韦林为对照药物,检测木芙蓉叶提取物抗RSV的活性。通过观察木芙蓉叶提取物不同给药方式的抗病毒作用,对木芙蓉叶提取物抗RSV的作用机制进行初探。结果:木芙蓉叶提取物能有效抑制RSV引起的细胞病变,具有显著的抗病毒作用,治疗指数(therapeutic index,TI)为86.24。木芙蓉叶提取物对病毒的直接灭活作用,同时具有预防和治疗的作用。结论:木芙蓉叶提取物在体外具有显著的抗RSV作用,且其抗病毒作用发生在病毒穿入细胞后的阶段。

Objective:To study the anti-respiratory syncytial viruses(RSV)mechanism of extracts from Hibiscus mutabilis leaf in vitro.Methods:Elibavirin was used as the positive drug to detect the anti-RSV activity of extracts from Hibiscus mutabilis leaf.By observing the antiviral effect of the extracts of Hibiscus mutabilis leaf in different ways of administration,the anti-RSV mechanism of the extracts of Hibiscus mutabilis leaf was preliminarily explored.Results:Hibiscus mutabilis leaf extracts could effectively inhibit the cytopathic changes caused by RSV,and had significant antiviral effect.The therapeutic index was 86.24.Conclusion:Hibiscus mutabilis leaf extracts have significant anti-RSV effect in vitro,and their antiviral effect occurs after virus penetration.